LOCUS HUMPTCAA 3642 bp mRNA linear HUM 15-JUL-1993
DEFINITION Human papillary thyroid carcinoma-encoded protein mRNA, complete
cds.
ACCESSION M31213
VERSION M31213.1
KEYWORDS papillary thyroid carcinoma-encoded protein; proto-oncogene;
tyrosine kinase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3642)
AUTHORS Grieco,M., Santoro,M., Berlingieri,M.T., Melillo,R.M., Donghi,R.,
Bongarzone,I., Pierotti,M.A., Della Porta,G., Fusco,A. and
Vecchio,G.
TITLE PTC is a novel rearranged form of the ret proto-oncogene and is
frequently detected in vivo in human thyroid papillary carcinomas
JOURNAL Cell 60 (4), 557-563 (1990)
PUBMED 2406025
REFERENCE 2 (sites)
AUTHORS Jhiang,S.M., Chiu,I.M. and Mazzaferri,E.L.
TITLE An STS in the human PTC oncogene located at 10q11.2
JOURNAL Nucleic Acids Res. 19 (15), 4303 (1991)
PUBMED 1678508
COMMENT Original source text: Human epithelial thyroid carcinoma
transfected NIH/3T3 cell line, cDNA to mRNA, clones
lambda-[213,413].
PCR components: 10 ng of genomic DNA, 0.5 ng of each
oligonucleotide, 200 micro-M dNTPs,
2.5 units Taq polymerase (Cetus) in
50 micro-l 1XPCR buffer (50 mM KCl,
10 mM Tris-HCl, pH 8.3 (at 25degC),
1 mM MgCl-2)
PCR reaction: 94 degrees C for 3 minutes
35 cycles of
30' at 94 degrees C
1' at 55 degrees C
2' at 72 degrees C
7' at 72 degrees C
Draft entry and computer-readable sequence kindly submitted by
M.Grieco, 11-JAN-1990.
FEATURES Location/Qualifiers
source 1..3642
/db_xref="H-InvDB:HIT000195416"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="papillary thyroid carcinoma"
STS 50..326
/map="10q11.2"
primer_bind 50..72
CDS 52..1563
/note="papillary thyroid carcinoma-encoded protein"
/codon_start=1
/map="10q1.1.2"
/protein_id="AAA36524.1"
/translation="MADSASESDTDGAGGNSSSSAAMQSSCSSTSGGGGGGGGGGGGG
KSGGIVISPFRLEELTNRLASLQQENKVLKIELETYKLKCKALQEENRDLRKASVTIE
DPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRGGYTTVAVKMLKENASPSELRD
LLSEFNVLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGS
GGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRK
MKISDFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIV
TLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISK
DLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRALPSTWIENKL
YGMSDPNWPGESPVPLTRADGTNTGFPRYPNDSVYANWMLSPSAAKLMDTFDS"
primer_bind complement(309..326)
BASE COUNT 938 a 846 c 942 g 916 t
ORIGIN
1 gaattcggca gctcgaggct gctcctcctc ctttcccagc ccgccgcggc catggcggac
61 agcgccagcg agagcgacac ggacggggcg gggggcaaca gcagcagctc ggccgccatg
121 cagtcgtcct gctcgtcgac ctcgggcggc ggcggtggcg gcgggggagg cggcggcggt
181 gggaagtcgg ggggcattgt catctcgccg ttccgcctgg aggagctcac caaccgcctg
241 gcctcgctgc agcaagagaa caaggtgctg aagatagagc tggagaccta caaactgaag
301 tgcaaggcac tgcaggagga gaaccgcgac ctgcgcaaag ccagcgttac catcgaggat
361 ccaaagtggg aattccctcg gaagaacttg gttcttggaa aaactctagg agaaggggaa
421 tttggaaaag tggtcaaggc aacggccttc catctgaaag gcagaggagg gtacaccacg
481 gtggccgtga agatgctgaa agagaacgcc tccccgagtg agcttcgaga cctgctgtca
541 gagttcaacg tcctgaagca ggtcaaccac cctcatgtca tcaaattgta tggggcctgc
601 agccaggatg gcccgctcct cctcatcgtg gagtacgcca aatacggctc cctgcggggc
661 ttcctccgcg agagccgcaa agtggggcct ggctacctgg gcagtggagg cagccgcaac
721 tccagctccc tggaccaccc ggatgagcgg gccctcacca tgggcgacct catctcattt
781 gcctggcaga tctcacaggg gatgcagtat ctggccgaga tgaagctcgt tcatcgggac
841 ttggcagcca gaaacatcct ggtagctgag gggcggaaga tgaagatttc ggatttcggc
901 ttgtcccgag atgtttatga agaggattcg tacgtgaaga ggagccaggg tcggattcca
961 gttaaatgga tggcaattga atcccttttt gatcatatct acaccacgca aagtgatgta
1021 tggtcttttg gtgtcctgct gtgggagatc gtgaccctag ggggaaaccc ctatcctggg
1081 attcctcctg agcggctctt caaccttctg aagaccggcc accggatgga gaggccagac
1141 aactgcagcg aggagatgta ccgcctgatg ctgcaatgct ggaagcagga gccggacaaa
1201 aggccggtgt ttgcggacat cagcaaagac ctggagaaga tgatggttaa gaggagagac
1261 tacttggacc ttgcggcgtc cactccatct gactccctga tttatgacga cggcctctca
1321 gaggaggaga caccgctggt ggactgtaat aatgcccccc tccctcgagc cctcccttcc
1381 acatggattg aaaacaaact ctatggcatg tcagacccga actggcctgg agagagtcct
1441 gtaccactca cgagagctga tggcactaac actgggtttc caagatatcc aaatgatagt
1501 gtatatgcta actggatgct ttcaccctca gcggcaaaat taatggacac gtttgatagt
1561 taacatttct ttgtgaaagg taatggactc acaaggggaa gaaacatgct gagaatggaa
1621 agtctaccgg ccctttcttt gtgaacgtca cattggccga gccgtgttca gttcccaggt
1681 ggcagactcg tttttggtag tttgttttaa cttccaaggt ggttttactt ctgatagccg
1741 gtgattttcc ctcctagcag acatgccaca ccgggtaaga gctctgagtc ttagtggtta
1801 agcattcctt tctcttcagt gcccagcagc acccagtgtt ggtctgtgtc catcagtgac
1861 caccaacatt ctgtgttcac atgtgtgggt ccaacactta ctacctggtg tatgaaattg
1921 gacctgaact gttggatttt tctagttgcc gccaaacaag gcaaaaaaat ttaaacatga
1981 agcacacaca caaaaaaggc agtaggaaaa atgctggccc tgatgacctg tccttattca
2041 gaatgagaga ctgcgggggg ggcctggggg tagtgtcaat gccccctcca gtgctggagg
2101 ggaagagggg ccccgaggat gggcctgggc tcagcattcg agatcttgag aatgattttt
2161 ttttaatcat gcaacctttc cttaggaaga catttggttt tcatcatgat taagatgatt
2221 cctagattta gcacaatgga gagattccat gccatcttta ctatgtggat ggtggtatca
2281 gggaagaggg ctcacaagac acatttgtgc ccccgggccc accacatcat cctcacgtgt
2341 tcggtactga gcagccacta cccctgatga caacagtatg aagaaagggg gctgttggag
2401 tcccagaatt gctgacagca gagcctttgc tgctgtgaat cccacctgcc accttcctgc
2461 agcacacccc acagccaagt agaggggaaa cgagtggctc atcctacctg ttaggagcag
2521 gtagggcttg tactcacttt aatttgaatc ttatcaactt actcataaag ggacaggcta
2581 gctagctgtg ttagaagtag caatgacaat gaccaaggac tgctacacct ctgattacaa
2641 ttctgatgtg aaaaagatgg tgtttggctc ttatagagcc tgtgtgaaag gcccatggat
2701 cagctcttcc tgtgtttgta atttaatgct gctacaaggt gtttctgttt cttagattct
2761 gaccatgagt gataagcttc ttgtcattct tcattgcttg tttgtggtca cagatgcaca
2821 acactcctcc agtcttgtgg gggcagcttt tgggaagtct cagcagctct tctggctgtg
2881 ttgtcagcac tgtaacttcg cagaaaagag tcggattacc aaaacactgc ctgctcttca
2941 gacttaaagc actgatagga cttaaaatag tctcattcaa atactgtatt ttatataggc
3001 atttcacaaa aacagacaaa attgtggcat tttgtgaggc caaggcttgg atgcgtgtgt
3061 aatagagcct tatggtgtgt gcgcacacac cacgagggag agtttgaaaa atgcttattg
3121 gacacgtaac ctggctctaa tttgggctgt ttttcagata cactgtgata agttctttta
3181 caaatatcta tagacatggt aaacttttgg ttttcagata tgcttaatga tagtcttact
3241 aaatgcagaa ataagaataa actttctcaa attattaaaa atgcctacac agtaagtgtg
3301 aattgctgca acaggtttgt tctcaggagg gtaagaactc caggtctaaa cagctgaccc
3361 gagtatgggg aatttatccg ttaccaattt atccttgacc aataacctaa ttgtctattc
3421 cgtagttata aaagtcccca tccttattag ctctactgga attttcatac acggtaaatg
3481 cagaagttac taagtattaa gtattactga gtattaagta gtaatctgtc agttattaaa
3541 atttgtaaaa tctatttatg aaaggtcatt aaaccagatc atgttccttt ttttgtaatc
3601 aaggtgacta agaaaatcag ttctgtaaat aaaaatcatc cg
//