LOCUS LT727634 9455 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pSV529HIFNG, complete sequence. ACCESSION LT727634 VERSION LT727634.1 KEYWORDS . SOURCE Mammalian expression vector pSV529HIFNG ORGANISM Mammalian expression vector pSV529HIFNG other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9455) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..9455 /organism="Mammalian expression vector pSV529HIFNG" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2234." /db_xref="taxon:1945111" regulatory 101..382 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 339..414 /note="SV40 ori" misc_feature 453..698 /note="SV40 large T-antigen" CDS 453..977 /transl_table=11 /note="SV40 small t-antigen" /protein_id="SJL89043.1" /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW CDIIGQTTYRDLKL" misc_feature 699..1044 /note="intron" misc_feature 1045..2925 /note="SV40 large T-antigen" regulatory complement(2926..3022) /note="polyA" /regulatory_class="terminator" regulatory 2926..3022 /note="polyA" /regulatory_class="terminator" CDS complement(3365..3865) /transl_table=11 /note="hIFNG" /protein_id="SJL89044.1" /translation="MKYTSYILAFQLCIVLGSLGCYCQDPYVKEAENLKKYFNAGHSD VADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNV KFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFR GRRASQ" misc_feature complement(3962..4899) /note="intron" misc_feature complement(4900..5102) /note="5UTR" regulatory complement(5103..5398) /note="SV40 late promoter" /regulatory_class="promoter" regulatory 5154..5435 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 5392..5467 /note="SV40 ori" misc_feature 5506..5751 /note="SV40 large T-antigen" CDS 5506..6030 /transl_table=11 /note="SV40 small t-antigen" /protein_id="SJL89045.1" /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW CDIIGQTTYRDLKL" misc_feature 5752..6097 /note="intron" misc_feature 6098..7160 /note="part of SV40 large t-antigen" rep_origin complement(7444..7846) /note="pMB1 ori" CDS complement(8387..9247) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL89046.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2821 a 1894 c 2087 g 2653 t ORIGIN 1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag 61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt 121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat 481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga 541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag 601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc 661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc 721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta 781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga 841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac 901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca 961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg 1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg 1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat 1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac 1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga 1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa 1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata 1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa 1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt 1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag 1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca 1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa 1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat 1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa 1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa 1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata 1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta 1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac 2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca 2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg 2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat 2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg 2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta 2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct 2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag 2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct 2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc 2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa 2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat 2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg 2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct 2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact 2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 3061 tgtatcttat catgtctgga tcttgcttag gttggctgcc tagttggccc ctgagataaa 3121 gccttgtaat cacatagcct tgcctaatta gtcagaaaac aaaggattaa gtgagacagt 3181 cacaggatat aggaattata aataatacat atattaatag atattcattt tcattacaca 3241 aaagttgcta ttataaatac ttatttgatt gatgagtcta aaaatatatt ccccatataa 3301 ataatgttaa atattaataa atagatttag atttaaaatt caaatattgc aggcaggaca 3361 accattactg ggatgctctt cgacctcgaa acagcatctg actccttttt cgcttccctg 3421 ttttagctgc tggcgacagt tcagccatca cttggatgag ttcatgtatt gctttgcgtt 3481 ggacattcaa gtcagttacc gaataattag tcagcttttc gaagtcatct cgtttctttt 3541 tgttgctatt gaaaaacttg acattcatgt cttccttgat ggtctccaca ctcttttgga 3601 tgctctggtc atctttaaag tttttaaaaa gtttgaagta aaaggagaca atttggctct 3661 gcattatttt tctgtcactc tcctctttcc aattcttcaa aatgcctaag aaaagagttc 3721 cattatccgc tacatctgaa tgacctgcat taaaatattt cttaaggttt tctgcttctt 3781 ttacatatgg gtcctggcag taacagccaa gagaacccaa aacgatgcag agctgaaaag 3841 ccaagatata acttgtatat ttcatcgttt ccgagagaat taagccaaag aagttgaaat 3901 cagtagttct tgtatcaagc tgatccggag cttttagagc agaagtaaca cttccgtaca 3961 ggcctagaag taaaggcaac atccactgag gagcagttct ttgatttgca ccaccaggag 4021 cctcaaattt ttcaataaat tcacctgact gcacattagg actttggctt tgagcttccc 4081 acctctcagt tacttgctga atactgtctg cttcatcaat attatcatag gtgtgcccaa 4141 atgatatttg caacccttcc ctgttggcta cttgtctcac cattgtaggc ctaatgggag 4201 acaaagtaga gtagtaatct tgtaaagagt tataccaatt aacaggagca ttaattactg 4261 tccaagtagt ttcctctaaa aaccttgcca aactgtccct taaatatctt tgggttcttc 4321 tttcaagctc ctgtgaggtg agcctaggaa tgtcattttg tattacacgc caaaaagctt 4381 gagaaatggc attaaaaagt gttggacccc aatgtctggg gtcaagatac tgaacactgt 4441 gaacaaaggt ttgtactcca ggaaataaaa tatcatagta atcatctggc ctatacaaat 4501 ctacagccat tcctggttgt tgatataaac caacagtaga aactttgtga tcccagtcac 4561 taaaaaatct ataccccact tgagcaacag cgctcacacc agtcacagtt tgcagtaaag 4621 ctgcaaatcc agctatagca gcaggagccc cagatatcac agcataggcc tgtggagtga 4681 ggcctatagc agcaattgcc tcagaggttg ttaggccttc aacagtagca acagatgcaa 4741 gttgcacttc aattgcagca gcggcctctc cagcagcaat ttcagctact gaaaatccag 4801 tagcagcagc agcttcagac acagtagcaa ttaggtcccc caacagtgtt aaagcagcac 4861 ccatggacct gaaataaaag acaaaaagac taaacttacc agttaacttt ctggtttttc 4921 agttaacctt tctggttttt tgcgtttccc gtcaacagta tcttcccctt cacaaaattg 4981 cagcaaaagc tctaaaacaa acacaaaaag gcgttgagct gtttttttac tttcagtcca 5041 tgacctacga accttaacgg aggcctggcg tgacagccgg cgcagcacca tggcctgaaa 5101 taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga 5161 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 5221 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 5281 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 5341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 5401 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 5461 gaggcttttt tggaggccta ggcttttgca aaaagctttg caaagatgga taaagtttta 5521 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgcctggggg 5581 aatattcctc tgatgagaaa ggcatattta aaaaaatgca aggagtttca tcctgataaa 5641 ggaggagatg aagaaaaaat gaagaaaatg aatactctgt acaagaaaat ggaagatgga 5701 gtaaaatatg ctcatcaacc tgactttgga ggcttctggg atgcaactga ggtatttgct 5761 tcttccttaa atcctggtgt tgatgcaatg tactgcaaac aatggcctga gtgtgcaaag 5821 aaaatgtctg ctaactgcat atgcttgctg tgcttactga ggatgaagca tgaaaataga 5881 aaattataca ggaaagatcc acttgtgtgg gttgattgct actgcttcga ttgctttaga 5941 atgtggtttg gacttgatct ttgtgaagga accttacttc tgtggtgtga cataattgga 6001 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg 6061 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga 6121 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc 6181 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag 6241 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt 6301 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact 6361 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta 6421 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa 6481 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata 6541 tttgatgtat agtgccttga ctagagatcc attttctgtt attgaggaaa gtttgccagg 6601 tgggttaaag gagcatgatt ttaatccaga agaagcagag gaaactaaac aagtgtcctg 6661 gaagcttgta acagagtatg caatggaaac aaaatgtgat gatgtgttgt tattgcttgg 6721 gatgtacttg gaatttcagt acagttttga aatgtgttta aaatgtatta aaaaagaaca 6781 gcccagccac tataagtacc atgaaaagca ttatgcaaat gctgctatat ttgctgacag 6841 caaaaaccaa aaaaccatat gccaacaggc tgttgatact gttttagcta aaaagcgggt 6901 tgatagccta caattaacta gagaacaaat gttaacaaac agatttaatg atcttttgga 6961 taggatggat ataatgtttg gttctacagg ctctgctgac atagaagaat ggatggctgg 7021 agttgcttgg ctacactgtt tgttgcccaa aatggattca gtggtgtatg actttttaaa 7081 atgcatggtg tacaacattc ctaaaaaaag atactggctg tttaaaggac caattgatag 7141 tggtaaaact acattagcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 7201 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 7261 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 7321 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga 7381 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 7441 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 7501 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 7561 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 7621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 7681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 7741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 7801 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 7861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 7921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 7981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 8041 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 8101 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 8161 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 8221 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 8281 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 8341 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 8401 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 8461 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 8521 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 8581 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 8641 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 8701 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 8761 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 8821 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 8881 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 8941 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 9001 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 9061 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 9121 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 9181 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 9241 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 9301 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 9361 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 9421 ataggcgtat cacgaggccc tttcgtcttc aagaa //