LOCUS LT727550 5962 bp DNA circular SYN 06-FEB-2017 DEFINITION Vector pLT10T3A20, complete sequence. ACCESSION LT727550 VERSION LT727550.1 KEYWORDS . SOURCE Vector pLT10T3A20 ORGANISM Vector pLT10T3A20 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5962) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5962 /organism="Vector pLT10T3A20" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2862." /db_xref="taxon:1945330" regulatory 24..40 /note="T7" /regulatory_class="promoter" misc_feature 41..103 /note="5UTR" CDS 104..2476 /transl_table=11 /note="hA20" /protein_id="SJL88815.1" /translation="MAEQVLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMH RYTLEMFRTCQFCPQFREIIHKALIDRNIQATLESQKKLNWCREVRKLVALKTNGDGN CLMHATSQYMWGVQDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD TRNWNDEWDNLIKMASTDTPMARSGLQYNSLEEIHIFVLCNILRRPIIVISDKMLRSL ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLV NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINAAKLDEAN LPKEINLVDDYFELVQHEYKKWQENSEQGRREGHAQNPMEPSVPQLSLMDVKCETPNC PFFMSVNTQPLCHECSERRQKNQNKLPKLNSKPGPEGLPGMALGASRGEAYEPLAWNP EESTGGPHSAPPTAPSPFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHNARQLHASH APDHTRHLDPGKCQACLQDVTRTFNGICSTCFKRTTAEASSSLSTSLPPSCHQRSKSD PSRLVRSPSPHSCHRAGNDAPAGCLSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFC TLCFIEYRENKHFAAASGKVSPTASRFQNTIPCLGRECGTLGSTMFEGYCQKCFIEAQ NQRFHEAKRTEEQLRSSQRRDVPRTTQSTSRPKCARASCKNILACRSEELCMECQHPN QRMGPGAHRGEPAPEDPPKQRCRAPACDHFGNAKCNGYCNECFQFKQMYG" regulatory complement(2557..2574) /note="T3" /regulatory_class="promoter" regulatory 2591..2638 /note="T7T" /regulatory_class="terminator" regulatory 2712..2759 /note="T7T" /regulatory_class="terminator" regulatory 2828..2909 /note="fdT" /regulatory_class="terminator" regulatory 2938..3019 /note="fdT" /regulatory_class="terminator" rep_origin 3117..3572 /note="f1 ori" rep_origin complement(3576..3949) /note="pMB1 ori" CDS complement(4490..5350) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88816.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 5716..5847 /note="PL" /regulatory_class="promoter" BASE COUNT 1568 a 1529 c 1438 g 1427 t ORIGIN 1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 61 ctctagaaat aattttgttt aactttaaga aggagatata catatggctg aacaagtcct 121 tcctcaggct ttgtatttga gcaatatgcg gaaagctgtg aagatacggg agagaactcc 181 agaagacatt tttaaaccta ctaatgggat cattcatcat tttaaaacca tgcaccgata 241 cacactggaa atgttcagaa cttgccagtt ttgtcctcag tttcgggaga tcatccacaa 301 agccctcatc gacagaaaca tccaggccac cctggaaagc cagaagaaac tcaactggtg 361 tcgagaagtc cggaagcttg tggcgctgaa aacgaacggt gacggcaatt gcctcatgca 421 tgccacttct cagtacatgt ggggcgttca ggacacagac ttggtactga ggaaggcgct 481 gttcagcacg ctcaaggaaa cagacacacg caactttaaa ttccgctggc aactggagtc 541 tctcaaatct caggaatttg ttgaaacggg gctttgctat gatactcgga actggaatga 601 tgaatgggac aatcttatca aaatggcttc cacagacaca cccatggccc gaagtggact 661 tcagtacaac tcactggaag aaatacacat atttgtcctt tgcaacatcc tcagaaggcc 721 aatcattgtc atttcagaca aaatgctaag aagtttggaa tcaggttcca atttcgcccc 781 tttgaaagtg ggtggaattt acttgcctct ccactggcct gcccaggaat gctacagata 841 ccccattgtt ctcggctatg acagccatca ttttgtaccc ttggtgaccc tgaaggacag 901 tgggcctgaa atccgagctg ttccacttgt taacagagac cggggaagat ttgaagactt 961 aaaagttcac tttttgacag atcctgaaaa tgagatgaag gagaagctct taaaagagta 1021 cttaatggtg atagaaatcc ccgtccaagg ctgggaccat ggcacaactc atctcatcaa 1081 tgccgcaaag ttggatgaag ctaacttacc aaaagaaatc aatctggtag atgattactt 1141 tgaacttgtt cagcatgagt acaagaaatg gcaggaaaac agcgagcagg ggaggagaga 1201 ggggcacgcc cagaatccca tggaaccttc cgtgccccag ctttctctca tggatgtaaa 1261 atgtgaaacg cccaactgcc ccttcttcat gtctgtgaac acccagcctt tatgccatga 1321 gtgctcagag aggcggcaaa agaatcaaaa caaactccca aagctgaact ccaagccggg 1381 ccctgagggg ctccctggca tggcgctcgg ggcctctcgg ggagaagcct atgagccctt 1441 ggcgtggaac cctgaggagt ccactggggg gcctcattcg gccccaccga cagcacccag 1501 cccttttctg ttcagtgaga ccactgccat gaagtgcagg agccccggct gccccttcac 1561 actgaatgtg cagcacaacg gattttgtga acgttgccac aacgcccggc aacttcacgc 1621 cagccacgcc ccagaccaca caaggcactt ggatcccggg aagtgccaag cctgcctcca 1681 ggatgttacc aggacattta atgggatctg cagtacttgc ttcaaaagga ctacagcaga 1741 ggcctcctcc agcctcagca ccagcctccc tccttcctgt caccagcgtt ccaagtcaga 1801 tccctcgcgg ctcgtccgga gcccctcccc gcattcttgc cacagagctg gaaacgacgc 1861 ccctgctggc tgcctgtctc aagctgcacg gactcctggg gacaggacgg ggacgagcaa 1921 gtgcagaaaa gccggctgcg tgtattttgg gactccagaa aacaagggct tttgcacact 1981 gtgtttcatc gagtacagag aaaacaaaca ttttgctgct gcctcaggga aagtcagtcc 2041 cacagcgtcc aggttccaga acaccattcc gtgcctgggg agggaatgcg gcacccttgg 2101 aagcaccatg tttgaaggat actgccagaa gtgtttcatt gaagctcaga atcagagatt 2161 tcatgaggcc aaaaggacag aagagcaact gagatcgagc cagcgcagag atgtgcctcg 2221 aaccacacaa agcacctcaa ggcccaagtg cgcccgggcc tcctgcaaga acatcctggc 2281 ctgccgcagc gaggagctct gcatggagtg tcagcatccc aaccagagga tgggccctgg 2341 ggcccaccgg ggtgagcctg cccccgaaga cccccccaag cagcgttgcc gggcccccgc 2401 ctgtgatcat tttggcaatg ccaagtgcaa cggctactgc aacgaatgct ttcagttcaa 2461 gcagatgtat ggctaaccgg aaacaggtgg gtcacctcct gcaagaagtg gggcctcgag 2521 gtcgacgaat tcgagctcgg ccgacttggc cttcccttta gtgagggtta ataaacttgg 2581 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct 2641 gaaaggagga actatatgcg ctcatacgat atgaacgttg agactgccgc tgagttatca 2701 gtgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc 2761 tgaaaggagg aactatatcc ggatagctta tcgctagagg tcgaaattca cctcgaaagc 2821 aagctgataa accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc 2881 aacgtgaaaa aattattatt cgcaattcca agctaattca cctcgaaagc aagctgataa 2941 accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc aacgtgaaaa 3001 aattattatt cgcaattcca agctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct 3061 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg cagatcacgc 3121 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 3181 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 3241 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 3301 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 3361 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 3421 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 3481 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 3541 gaattttaac aaaatattaa cgtttacaat ttgatctgcg ctcggtcgtt cggctgcggc 3601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 3661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 3721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 3781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 3841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 3901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 3961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 4021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 4081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 4141 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 4201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 4261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 4321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 4381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 4441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 4501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 4561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 4621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 4681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 4741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 4801 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 4861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 4921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 4981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 5041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 5101 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 5161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 5221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 5281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 5341 gaatactcat actcttcctt tttcaatatt atgtaagcag acagttttat tgttcatgat 5401 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt 5461 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca 5521 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct 5581 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat 5641 gagtcagcaa caccttcttc acgaggcaga cctcagcgcc ggtgatgccg gccacgatgc 5701 gtccggcgta gaggatctct cacctaccaa acaatgcccc cctgcaaaaa ataaattcat 5761 ataaaaaaca tacagataac catctgcggt gataaattat ctctggcggt gttgacataa 5821 ataccactgg cggtgatact gagcacatca gcaggacgca ctgaccacca tgaaggtgac 5881 gctcttaaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt 5941 gatacgaaac gaagcattgg aa //