LOCUS LT727418 4740 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector pBLmIL6CAT, complete sequence.
ACCESSION LT727418
VERSION LT727418.1
KEYWORDS .
SOURCE Reporter vector pBLmIL6CAT
ORGANISM Reporter vector pBLmIL6CAT
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4740)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4740
/organism="Reporter vector pBLmIL6CAT"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2988."
/db_xref="taxon:1945202"
regulatory 42..519
/note="mIL6"
/regulatory_class="promoter"
CDS 578..1237
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88385.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS <1327..1401
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88386.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 1402..1468
/note="intron"
misc_feature 1469..2020
/note="3UTR"
regulatory 2021..2117
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2021..2117)
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2256..2339)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(2431..2833)
/note="pMB1 ori"
CDS complement(3374..4234)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88387.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4366..4440
/note="polyA"
/regulatory_class="terminator"
regulatory complement(4366..4440)
/note="polyA"
/regulatory_class="terminator"
regulatory 4581..4677
/note="polyA"
/regulatory_class="terminator"
regulatory complement(4581..4677)
/note="polyA"
/regulatory_class="terminator"
BASE COUNT 1285 a 1060 c 1028 g 1367 t
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatccttac agacatacaa aagaatccta
61 gcctcttatt catgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta
121 tgtgtgtgtc gtctgtcatg cgcgcgtgcc tgcgtttaaa taacatcagc tttacgttct
181 ctttctcctt ataaaacatt gtgaatttca gttttctttc ccatcaagac atgctcaagt
241 gctgagtcac ttttaaagaa aaaaagaaga gtgctcatgc ttcttagggc tagcctcaag
301 gatgacttaa gcacactttt cccttcctag ttgtgattct ttcgatgcta aacgacgtca
361 cattgtgcaa tcttaataag gtttccaatc agccccaccc actctggccc cacccccacc
421 ctccaacaaa gatttttatc aaatgtggga ttttcccatg agtctcaaaa ttagagagtt
481 gactcctaat aaatatgaga ctggggatgt ctgtagctca ttctgctctg ggatctcgag
541 gagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
601 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
661 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
721 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
781 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
841 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
901 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
961 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
1021 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
1081 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
1141 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
1201 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
1261 cccttaaacg cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
1321 tcgccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
1381 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
1441 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
1501 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
1561 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
1621 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
1681 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
1741 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
1801 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
1861 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
1921 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
1981 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
2041 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
2101 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
2161 cttatcatgt ctggatcgat ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc
2221 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
2281 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
2341 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
2401 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
2461 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
2521 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
2581 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2641 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2701 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2761 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
2821 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
2881 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
2941 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
3001 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
3061 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
3121 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
3181 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3241 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3301 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3361 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
3421 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
3481 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
3541 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
3601 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
3661 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
3721 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
3781 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
3841 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
3901 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
3961 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
4021 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
4081 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
4141 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
4201 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
4261 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4321 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaactt gtttattgca
4381 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
4441 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
4501 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
4561 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
4621 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
4681 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctcg
//