LOCUS LT727329 7171 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector LNXRO2, complete sequence.
ACCESSION LT727329
VERSION LT727329.1
KEYWORDS .
SOURCE Mammalian expression vector LNXRO2
ORGANISM Mammalian expression vector LNXRO2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7171)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7171
/organism="Mammalian expression vector LNXRO2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4227."
/db_xref="taxon:1944744"
regulatory 145..733
/note="5LTR"
/regulatory_class="promoter"
CDS 1656..2450
/transl_table=11
/note="neomycin resistance"
/protein_id="SJL88127.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory complement(2950..3121)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(2950..3864)
/note="RO2"
misc_feature complement(3185..3517)
/note="intron"
regulatory complement(3559..3864)
/note="RSV"
/regulatory_class="promoter"
misc_feature 4217..4810
/note="3LTR"
rep_origin complement(5165..5565)
/note="pMB1 ori"
CDS complement(6106..6966)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88128.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1652 a 1979 c 1813 g 1727 t
ORIGIN
1 gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
61 tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
121 ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
181 aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
241 caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
301 tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
361 tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
421 cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
481 aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
541 cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
601 cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
661 gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
721 ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
781 ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
841 tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
901 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
961 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
1021 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
1081 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
1141 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
1201 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
1261 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
1321 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
1381 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
1441 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
1501 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
1561 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
1621 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
1681 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
1741 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
1801 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
1861 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
1921 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
1981 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
2041 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
2101 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
2161 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
2221 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
2281 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
2341 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
2401 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
2461 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
2521 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
2581 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
2641 gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
2701 atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
2761 ggagtgggga ggcacgatgg ccgctttggt cgaggcggat cctagccccc acgaaccata
2821 aaccattccc catgggggac cccgtcccta acccacgggg ccagtggcta tggcagggcc
2881 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
2941 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
3001 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
3061 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
3121 ttcagttctt aagaattgcg gccgcgatcg cgttaacaga tctggccgca agcttggttg
3181 gaatctaaaa tacacaaaca attaggatcc ctgaggagca gttctttgaa ctaggttgtg
3241 gaattgtgag cgctcacaat tccacagtcg accctagtcc accaggagcc tcaaattttt
3301 caataaattc acctgactgc acattaggac tttggctttg agcttcccac ctctcagtta
3361 cttgctgaat actgtctgct tcatcaatat tatcataggt gtgcccaaat gatatttgca
3421 acccttccct gttggctact tgtctcacga ttgtaggcct aatgggagac ggatccgtag
3481 tttaacacat tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg
3541 tagtttgtcc aagcttggag gtgcacacca atgtggtgaa tggtcaaatg gcgtttattg
3601 tatactaggg tcgactgtgg aattgtgagc gctcacaatt ccacaaccta gtaggcactt
3661 aaatacaata tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacccgtc
3721 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcaccc
3781 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
3841 aagagtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc
3901 tgaggggact cctaaccgcg tacaaccgaa gccccgcttt tcgcctaaac acaccctagt
3961 cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
4021 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
4081 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
4141 cggcatcaga gcagattgta ctgccaccaa catcgataaa ataaaagatt ttatttagtc
4201 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
4261 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt
4321 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
4381 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
4441 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
4501 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
4561 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
4621 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
4681 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
4741 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
4801 gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca
4861 ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
4921 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
4981 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
5041 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
5101 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
5161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
5221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
5281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
5341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
5401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
5461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
5521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
5581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
5641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
5701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
5761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
5821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
5881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
5941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
6001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
6061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
6121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
6181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
6241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
6301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
6361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
6421 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
6481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
6541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
6601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
6661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
6721 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
6781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
6841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
6901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
6961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
7021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
7081 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
7141 taggcgtatc acgaggccct ttcgtcttca a
//