LOCUS LT727317 10878 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pPIC9-TSE, complete sequence.
ACCESSION LT727317
VERSION LT727317.1
KEYWORDS .
SOURCE Yeast expression vector pPIC9-TSE
ORGANISM Yeast expression vector pPIC9-TSE
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 10878)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..10878
/organism="Yeast expression vector pPIC9-TSE"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4166."
/db_xref="taxon:1945490"
regulatory 7..772
/note="AOX1 promoter"
/regulatory_class="promoter"
misc_feature 773..937
/note="5' UTR of AOX1"
CDS 949..>1191
/transl_table=11
/note="ppMF"
/protein_id="SJL88088.1"
/translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDL
EGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVS"
CDS 1219..>3141
/transl_table=11
/note="mature TS"
/protein_id="SJL88089.1"
/translation="MLAPGSSRVELFKRKNSTVPFEDKAGKVTERVVHSFRLPALVNV
DGVMVAIADARYDTSNDNSLIDTVAKYSVDDGETWETQIAIKNSRVSSVSRVVDPTVI
VKGNKLYVLVGSYYSSRSYWSSHGDARDWDILLAVGEVTKSIAGGKITASIKWGSPVS
LKKFFPAEMEGMHTNQFLGGAGVAIVASNGNLVYPVQVTNKRKQVFSKIFYSEDDGKT
WKFGKGRSDFGCSEPVALEWEGKLIINTRVDWKRRLVYESSDMGNTWVEAVGTLSRVW
GPSPKSDHPGSQSSFTAVTIEGMRVMLFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQ
VSIGDENSAYSSVLYKDDKLYCLHEINTDEVYSLVFARLVGELRIIKSVLRSWKNWDS
HLSSICTPADPAASSSESGCGPAVTTVGLVGFLSGNASQNVWEDAYRCVNASTANAER
VRNGLKFAGVGGGALWPVSQQGQNQRYRFANHAFTLVASVTIHEAPRAASPLLGASLD
SSGGKKLLGLSYDEKHQWQPIYGSTPVTPTGSWETGKRYHVVLTVANKIGSVYIDGEL
LEGSGQTVVPDGRTPDISHFYVGGYGRSDMPTISHVTVNNVLLYNRQLNTEEIRTLFL
SQDLIGTEAHMDSSSDT"
misc_feature 3151..3189
/note="E-tag"
CDS <3199..>4090
/codon_start=1
/transl_table=11
/note="fd g3 fragment"
/protein_id="SJL88090.1"
/translation="TVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVVVC
TGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYIN
PLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPV
KTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGG
SGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADEN
ALQSDAKGKLDSVATDYGAAI"
regulatory 4176..4435
/note="AOX1 terminator"
/regulatory_class="terminator"
CDS complement(<4838..7369)
/transl_table=11
/note="Pichia HIS4"
/protein_id="SJL88091.1"
/translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST
ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR
TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII
PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS
KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF
CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE
AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ
PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ
NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLT
ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP
PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA
AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAV
DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI
LQIENASSYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQ
KFITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
misc_feature 7724..8481
/note="3' flanking region of AOX1"
rep_origin complement(8765..9167)
/note="pMB1 ori"
CDS complement(9708..10568)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88092.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2788 a 2568 c 2663 g 2859 t
ORIGIN
1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
1201 agagaggctg aagcttacgt gctggcaccc ggatcgagcc gagttgagct gtttaagcgt
1261 aagaattcga cggtgccgtt tgaagacaag gccggcaaag tcaccgagcg ggttgtccac
1321 tcgttccgcc tccccgccct tgttaatgtg gacggggtga tggttgccat cgcggacgct
1381 cgctacgaca catccaatga caactccctc attgatacgg tggcgaagta cagcgtggac
1441 gatggggaga cgtgggagac ccaaattgcc atcaagaaca gccgtgtatc gtctgtttct
1501 cgtgtggtgg atcccaccgt gattgtgaag ggcaacaagc tttacgtcct ggttggaagc
1561 tactatagtt cgagaagcta ctggtcgtcg catggtgatg cgagagactg ggatattctg
1621 cttgccgttg gtgaggtcac gaagtccatt gcgggcggca agataactgc gagtatcaaa
1681 tgggggagcc ccgtgtcact gaagaagttt tttccggcag aaatggaagg catgcacaca
1741 aatcaatttc ttggcggcgc gggtgttgcc attgtggcgt ccaacgggaa tcttgtgtac
1801 cctgtgcagg ttacgaacaa aaggaagcaa gttttctcca agatcttcta ctcggaagat
1861 gatgggaaga cgtggaagtt tgggaagggt aggagcgatt ttggctgctc tgaacctgtg
1921 gcccttgagt gggaggggaa gctcatcata aacacccgag ttgactggaa acgccgtctg
1981 gtgtacgagt ccagtgacat ggggaatacg tgggtggagg ctgtcggcac gctctcgcgt
2041 gtgtggggcc cctcaccaaa atcggaccat cccggcagtc agagcagctt cactgccgtg
2101 accatcgaag gaatgcgtgt gatgctcttc acacacccgc tgaattttaa gggaaggtgg
2161 ctgcgcgacc gactgaacct ctggctgacg gataaccagc gcatttataa cgttgggcaa
2221 gtatccattg gtgatgaaaa ttccgcctac agctccgtcc tgtacaagga tgataagctg
2281 tactgtttgc atgagatcaa cacggacgag gtgtacagcc ttgtttttgc acgcctggtt
2341 ggcgagctac ggatcattaa atcagtgctg cgatcctgga agaattggga cagccacctg
2401 tccagcattt gcacccctgc tgatccagcc gcttcgtcgt cagagagtgg ttgtggtccc
2461 gctgtcacca cggttggtct tgttggcttt ttgtccggca atgcctccca aaacgtatgg
2521 gaggatgcgt accgctgcgt caacgcaagc acggcaaatg cggagagggt tcggaacggt
2581 ttgaagtttg cgggggttgg cggaggggcg ctttggccgg tgagccagca ggggcagaat
2641 cagcggtatc gttttgcaaa ccacgcgttc acgctggtgg cgtcggtgac gattcacgag
2701 gctccgaggg ccgcgagtcc tttgctgggt gcgagcctgg actcttctgg tggcaaaaaa
2761 ctcctggggc tctcgtacga cgagaagcac cagtggcagc caatatacgg atcaacgccg
2821 gtgacgccga cgggatcgtg ggagacgggt aagaggtacc acgtggttct tacggtggcg
2881 aataaaattg gctccgtgta cattgatgga gaacttctgg agggttcagg acagaccgtt
2941 gtgccagacg ggaggacgcc tgacatctcc cacttctacg ttggcgggta tggaaggagt
3001 gatatgccaa ccataagcca cgtgacggtg aataatgttc ttctttacaa ccgacagctg
3061 aataccgagg agatcaggac cttgttcttg agccaggacc tgattggcac ggaagcacac
3121 atggacagca gcagcgacac gaaggaattg ggtgcgccgg tgccgtatcc ggatccgctg
3181 gaaccgcgtg ccgcatagac tgttgaaagt tgtttagcaa aacctcatac agaaaattca
3241 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt
3301 ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg aaactcagtg ttacggtaca
3361 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
3421 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
3481 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
3541 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
3601 cagaataata ggttccgaaa taggcagggt gcattaactg tttatacggg cactgttact
3661 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
3721 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
3781 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
3841 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggcgg ctctgagggt
3901 ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt
3961 gattttgatt atgaaaaaat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
4021 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
4081 gctgctatcg ggccgcgaat taattcgcct tagacatgac tgttcctcag ttcaagttgg
4141 gcacttacga gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt
4201 gcctgagaga tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg
4261 attttttttg tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca
4321 gctgatgaat atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg
4381 gtatttccca ctcctcttca gagtacagaa gattaagtga gaagttcgtt tgtgcaagct
4441 tatcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc
4501 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt
4561 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga
4621 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
4681 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
4741 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcta
4801 tcgaatctaa atgtaagtta aaatctctaa ataattaaat aagtcccagt ttctccatac
4861 gaaccttaac agcattgcgg tgagcatcta gaccttcaac agcagccaga tccatcactg
4921 cttggccaat atgtttcagt ccctcaggag ttacgtcttg tgaagtgatg aacttctgga
4981 aggttgcagt gttaactccg ctgtattgac gggcatatcc gtacgttggc aaagtgtggt
5041 tggtaccgga ggagtaatct ccacaactct ctggagagta ggcaccaaca aacacagatc
5101 cagcgtgttg tacttgatca acataagaag aagcattctc gatttgcagg atcaagtgtt
5161 caggagcgta ctgattggac atttccaaag cctgctcgta ggttgcaacc gatagggttg
5221 tagagtgtgc aatacacttg cgtacaattt caacccttgg caactgcaca gcttggttgt
5281 gaacagcatc ttcaattctg gcaagctcct tgtctgtcat atcgacagcc aacagaatca
5341 cctgggaatc aataccatgt tcagcttgag acagaaggtc tgaggcaacg aaatctggat
5401 cagcgtattt atcagcaata actagaactt cagaaggccc agcaggcatg tcaatactac
5461 acagggctga tgtgtcattt tgaaccatca tcttggcagc agtaacgaac tggtttcctg
5521 gaccaaatat tttgtcacac ttaggaacag tttctgttcc gtaagccata gcagctactg
5581 cctgggcgcc tcctgctagc acgatacact tagcaccaac cttgtgggca acgtagatga
5641 cttctggggt aagggtacca tccttcttag gtggagatgc aaaaacaatt tctttgcaac
5701 cagcaacttt ggcaggaaca cccagcatca gggaagtgga aggcagaatt gcggttccac
5761 caggaatata gaggccaact ttctcaatag gtcttgcaaa acgagagcag actacaccag
5821 ggcaagtctc aacttgcaac gtctccgtta gttgagcttc atggaatttc ctgacgttat
5881 ctatagagag atcaatggct ctcttaacgt tatctggcaa ttgcataagt tcctctggga
5941 aaggagcttc taacacaggt gtcttcaaag cgactccatc aaacttggca gttagttcta
6001 aaagggcttt gtcaccattt tgacgaacat tgtcgacaat tggtttgact aattccataa
6061 tctgttccgt tttctggata ggacgacgaa gggcatcttc aatttcttgt gaggaggcct
6121 tagaaacgtc aattttgcac aattcaatac gaccttcaga agggacttct ttaggtttgg
6181 attcttcttt aggttgttcc ttggtgtatc ctggcttggc atctcctttc cttctagtga
6241 cctttaggga cttcatatcc aggtttctct ccacctcgtc caacgtcaca ccgtacttgg
6301 cacatctaac taatgcaaaa taaaataagt cagcacattc ccaggctata tcttccttgg
6361 atttagcttc tgcaagttca tcagcttcct ccctaatttt agcgttcaac aaaacttcgt
6421 cgtcaaataa ccgtttggta taagaacctt ctggagcatt gctcttacga tcccacaagg
6481 tggcttccat ggctctaaga ccctttgatt ggccaaaaca ggaagtgcgt tccaagtgac
6541 agaaaccaac acctgtttgt tcaaccacaa atttcaagca gtctccatca caatccaatt
6601 cgatacccag caacttttga gttgctccag atgtagcacc tttataccac aaaccgtgac
6661 gacgagattg gtagactcca gtttgtgtcc ttatagcctc cggaatagac tttttggacg
6721 agtacaccag gcccaacgag taattagaag agtcagccac caaagtagtg aatagaccat
6781 cggggcggtc agtagtcaaa gacgccaaca aaatttcact gacagggaac tttttgacat
6841 cttcagaaag ttcgtattca gtagtcaatt gccgagcatc aataatgggg attataccag
6901 aagcaacagt ggaagtcaca tctaccaact ttgcggtctc agaaaaagca taaacagttc
6961 tactaccgcc attagtgaaa cttttcaaat cgcccagtgg agaagaaaaa ggcacagcga
7021 tactagcatt agcgggcaag gatgcaactt tatcaaccag ggtcctatag ataaccctag
7081 cgcctgggat catcctttgg acaactcttt ctgccaaatc taggtccaaa atcacttcat
7141 tgataccatt attgtacaac ttgagcaagt tgtcgatcag ctcctcaaat tggtcctctg
7201 taacggatga ctcaacttgc acattaactt gaagctcagt cgattgagtg aacttgatca
7261 ggttgtgcag ctggtcagca gcatagggaa acacggcttt tcctaccaaa ctcaaggaat
7321 tatcaaactc tgcaacactt gcgtatgcag gtagcaaggg aaatgtcata cttgaagtcg
7381 gacagtgagt gtagtcttga gaaattctga agccgtattt ttattatcag tgagtcagtc
7441 atcaggagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
7501 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
7561 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
7621 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
7681 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagta tctatgattg
7741 gaagtatggg aatggtgata cccgcattct tcagtgtctt gaggtctcct atcagattat
7801 gcccaactaa agcaaccgga ggaggagatt tcatggtaaa tttctctgac ttttggtcat
7861 cagtagactc gaactgtgag actatctcgg ttatgacagc agaaatgtcc ttcttggaga
7921 cagtaaatga agtcccacca ataaagaaat ccttgttatc aggaacaaac ttcttgtttc
7981 gaactttttc ggtgccttga actataaaat gtagagtgga tatgtcgggt aggaatggag
8041 cgggcaaatg cttaccttct ggaccttcaa gaggtatgta gggtttgtag atactgatgc
8101 caacttcagt gacaacgttg ctatttcgtt caaaccattc cgaatccaga gaaatcaaag
8161 ttgtttgtct actattgatc caagccagtg cggtcttgaa actgacaata gtgtgctcgt
8221 gttttgaggt catctttgta tgaataaatc tagtctttga tctaaataat cttgacgagc
8281 caaggcgata aatacccaaa tctaaaactc ttttaaaacg ttaaaaggac aagtatgtct
8341 gcctgtatta aaccccaaat cagctcgtag tctgatcctc atcaacttga ggggcactat
8401 cttgttttag agaaatttgc ggagatgcga tatcgagaaa aaggtacgct gattttaaac
8461 gtgaaattta tctcaagatc tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
8521 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
8581 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
8641 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
8701 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
8761 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
8821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
8881 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
8941 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
9001 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
9061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
9121 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
9181 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
9241 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
9301 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
9361 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
9421 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
9481 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
9541 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
9601 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
9661 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
9721 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
9781 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
9841 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
9901 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
9961 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
10021 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
10081 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
10141 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
10201 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
10261 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
10321 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
10381 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
10441 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
10501 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
10561 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
10621 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
10681 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
10741 aataggcgta tcacgaggcc ctttcgtctt caagaattaa ttctcatgtt tgacagctta
10801 tcatcgataa gctgactcat gttggtattg tgaaatagac gcagatcggg aacactgaaa
10861 aataacagtt attattcg
//