LOCUS LT727312 6178 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAGmA20f1, complete sequence.
ACCESSION LT727312
VERSION LT727312.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAGmA20f1
ORGANISM Mammalian expression vector pCAGGS-FLAGmA20f1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6178)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6178
/organism="Mammalian expression vector pCAGGS-FLAGmA20f1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4121."
/db_xref="taxon:1944854"
misc_feature 13..36
/note="FLAG epitope"
CDS 46..>1449
/transl_table=11
/note="mA20 (1-468)"
/protein_id="SJL88078.1"
/translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGN
CLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCETPNC
PFFMSVNTQPLCHECSERRQKNQSKLPKLNSKLGPEGLPGVGLGSSNWSPEETAGGPH
SAPPTAPSLFLFSETTAM"
regulatory 1464..1555
/note="3' UTR of beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1556..1912
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1990..2072)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2214..2289
/note="SV40 ori"
regulatory 2320..2394
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2320..2394)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2509..2910)
/note="pMB1 ori"
CDS complement(3451..4311)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88079.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4451..4828
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4834..6175
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5203..6127
/note="intron AG promoter"
BASE COUNT 1406 a 1616 c 1661 g 1495 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt
61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc
121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga
181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac
241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg
301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg
361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc
421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa
481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat
541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga
601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga
661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct
721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga
781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac
841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac
901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag
961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc
1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac
1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga
1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata
1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac
1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta
1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc
1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc
1441 actgcaatgt aagtgtcgac gaagatcttt ttccctctgc caaaaattat ggggacatca
1501 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
1561 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
1621 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
1681 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
1741 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
1801 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
1861 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
1921 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
1981 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg ggtgcctaat
2041 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
2101 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2161 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2221 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
2281 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
2341 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
2401 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
2461 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
2521 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
2581 caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc
2641 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
2701 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
2761 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
2821 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
2881 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
2941 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
3001 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
3061 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
3121 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
3181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
3241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
3301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
3361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
3421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
3481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
3541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
3601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
3661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
3721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
3781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
3841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
3901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
3961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
4021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
4081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
4141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
4201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
4261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
4321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
4381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
4441 tgggtcgatc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
4501 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
4561 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
4621 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
4681 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
4741 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
4801 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
4861 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
4921 tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc ggggcggggc
4981 gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc
5041 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
5101 cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg
5161 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
5221 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
5281 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
5341 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
5401 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
5461 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
5521 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
5581 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
5641 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
5701 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg gcggcccccg
5761 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
5821 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
5881 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
5941 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
6001 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
6061 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
6121 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaa
//