LOCUS LT727291 5872 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAGmA20f3, complete sequence.
ACCESSION LT727291
VERSION LT727291.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAGmA20f3
ORGANISM Mammalian expression vector pCAGGS-FLAGmA20f3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5872)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5872
/organism="Mammalian expression vector pCAGGS-FLAGmA20f3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3953."
/db_xref="taxon:1944856"
misc_feature 13..36
/note="FLAG epitope"
CDS 46..>1143
/transl_table=11
/note="mA20 (1-366)"
/protein_id="SJL88009.1"
/translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGN
CLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSDQARRA"
regulatory 1158..1249
/note="3' UTR of beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1250..1606
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1684..1766)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1908..1983
/note="SV40 ori"
regulatory 2014..2088
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2014..2088)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2203..2604)
/note="pMB1 ori"
CDS complement(3145..4005)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88010.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4145..4522
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4528..5869
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4897..5821
/note="intron AG promoter"
BASE COUNT 1325 a 1515 c 1595 g 1437 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt
61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc
121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga
181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac
241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg
301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg
361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc
421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa
481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat
541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga
601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga
661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct
721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga
781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac
841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac
901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag
961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc
1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac
1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga
1141 gcgtaagtgt cgacgaagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
1201 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
1261 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
1321 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
1381 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
1441 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
1501 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
1561 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
1621 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
1681 attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc taatgagtga
1741 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
1801 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
1861 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
1921 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
1981 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
2041 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
2101 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
2161 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
2221 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
2281 cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt gagcaaaagg
2341 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
2401 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
2461 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
2521 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
2581 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
2641 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
2701 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
2761 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
2821 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
2881 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
2941 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
3001 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
3061 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
3121 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
3181 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
3241 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
3301 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
3361 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
3421 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
3481 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
3541 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
3601 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
3661 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
3721 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
3781 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
3841 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
3901 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
3961 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
4021 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
4081 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
4141 gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
4201 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
4261 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
4321 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
4381 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
4441 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
4501 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
4561 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
4621 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
4681 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
4741 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
4801 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
4861 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
4921 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
4981 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
5041 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
5101 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
5161 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
5221 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
5281 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
5341 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
5401 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
5461 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
5521 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
5581 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
5641 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
5701 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
5761 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
5821 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
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