LOCUS LT727286 6079 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-EmCASP-1m1, complete sequence. ACCESSION LT727286 VERSION LT727286.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-EmCASP-1m1 ORGANISM Mammalian expression vector pCAGGS-EmCASP-1m1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6079) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6079 /organism="Mammalian expression vector pCAGGS-EmCASP-1m1" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3914." /db_xref="taxon:1944809" misc_feature 16..54 /note="E-tag" CDS <61..1266 /codon_start=1 /transl_table=11 /note="mouse CASP-1 mutant" /protein_id="SJL87997.1" /translation="ADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKIKL ANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAGILELQSAPSAETFVATEE SKGGHPSSSETKEEQNKEEGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTRT RLALIICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFAA CPEHKTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPK VIIIQACRGEKQGVVLLKDSVRDSEEDFLTDAIFEDDGIKKAHIEKDFIAFCSSTPDN VSWRHPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLT KRFYLFPGH" misc_feature 364..366 /note="D103E" misc_feature 421..423 /note="D122E" regulatory 1369..1460 /note="3' UTR of beta-globin, incl. polyA" /regulatory_class="terminator" misc_feature 1461..1817 /note="3' flanking region rabbit beta-globin" regulatory complement(1895..1977) /note="lac promoter" /regulatory_class="promoter" rep_origin 2119..2194 /note="SV40 ori" regulatory 2225..2299 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2225..2299) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(2414..2815) /note="pMB1 ori" CDS complement(3356..4216) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87998.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4352..4729 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4735..6076 /note="AG promoter" /regulatory_class="promoter" misc_feature 5104..6028 /note="intron AG promoter" BASE COUNT 1401 a 1530 c 1636 g 1512 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gctgacaaga tcctgagggc aaagaggaag caatttatca actcagtgag tatagggaca 121 ataaatggat tgttggatga acttttagag aagagagtgc tgaatcagga agaaatggat 181 aaaataaaac ttgcaaacat tactgctatg gacaaggcac gggacctatg tgatcatgtc 241 tctaaaaaag ggccccaggc aagccaaatc tttatcactt acatttgtaa tgaagactgc 301 tacctggcag gaattctgga gcttcaatca gctccatcag ctgaaacatt tgttgctaca 361 gaagaatcta aaggtggcca tccttcatcc tcagaaacaa aggaagaaca gaacaaagaa 421 gagggtacct ttccaggact gactgggacc ctcaagtttt gccctttaga aaaagcccag 481 aagttatgga aagaaaatcc ttcagagatt tatccaataa tgaatacaac cactcgtaca 541 cgtcttgccc tcattatctg caacacagag tttcaacatc tttctccgag ggttggagct 601 caagttgacc tcagagaaat gaagttgctg ctggaggatc tggggtatac cgtgaaagtg 661 aaagaaaatc tcacagctct ggagatggtg aaagaggtga aagaatttgc tgcctgccca 721 gagcacaaga cttctgacag tactttcctt gtattcatgt ctcatggtat ccaggaggga 781 atatgtggga ccacatactc taatgaagtt tcagatattt taaaggttga cacaatcttt 841 cagatgatga acactttgaa gtgcccaagc ttgaaagaca agcccaaggt gatcattatt 901 caggcatgcc gtggagagaa acaaggagtg gtgttgttaa aagattcagt aagagactct 961 gaagaggatt tcttaacgga tgcaattttt gaagatgatg gcattaagaa ggcccatata 1021 gagaaagatt ttattgcttt ctgctcttca acaccagata atgtgtcttg gagacatcct 1081 gtcaggggct cacttttcat tgagtcactc atcaaacaca tgaaagaata tgcctggtct 1141 tgtgacttgg aggacatttt cagaaaggtt cgattttcat ttgaacaacc agaatttagg 1201 ctacagatgc ccactgctga tagggtgacc ctgacaaaac gtttctacct cttcccggga 1261 cattaaacgc gtgggtaccg gatccggcaa ttcactcctc aggtgcaggc tgcctatcag 1321 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc 1381 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa 1441 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg 1501 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca 1561 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat 1621 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag 1681 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta 1741 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct 1801 cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct 1861 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 1921 aaagtgtaaa gcctgggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 1981 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag 2041 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 2101 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 2161 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 2221 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 2281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 2341 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 2401 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 2461 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 2521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 2581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 2641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 2701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 2761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 2821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 2881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 2941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 3001 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 3061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 3121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 3181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 3241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 3301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 3361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 3421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 3481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 3541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 3601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 3661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 3721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 3781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 3841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 3901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 3961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 4081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 4141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 4201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 4261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 4321 gcacatttcc ccgaaaagtg ccacctgggt cgacattgat tattgactag ttattaatag 4381 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 4441 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 4501 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 4561 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 4621 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 4681 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg 4741 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat 4801 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcccccc 4861 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca 4921 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg 4981 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg 5041 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca 5101 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga 5161 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 5221 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc 5281 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc 5341 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc 5401 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc 5461 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg 5521 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg 5581 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg 5641 gaaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca 5701 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc 5761 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg 5821 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc 5881 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg 5941 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca 6001 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc 6061 tcatcatttt ggcaaagaa //