LOCUS LT727259 6160 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian vector pdECFP, complete sequence.
ACCESSION LT727259
VERSION LT727259.1
KEYWORDS .
SOURCE Mammalian vector pdECFP
ORGANISM Mammalian vector pdECFP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6160)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6160
/organism="Mammalian vector pdECFP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4548."
/db_xref="taxon:1945175"
regulatory 1..568
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
CDS 613..>1329
/transl_table=11
/note="ECFP"
/protein_id="SJL87914.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
regulatory complement(1348..1472)
/note="attR1"
/regulatory_class="attenuator"
regulatory 1480..1532
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 1581..2240
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87915.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 2582..2887
/transl_table=11
/note="ccdB"
/protein_id="SJL87916.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 2928..3052
/note="attR2"
/regulatory_class="attenuator"
regulatory complement(3163..3259)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 3163..3259
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(3313..3768)
/note="f1 ori"
regulatory 3769..3864
/note="amp promoter"
/regulatory_class="promoter"
misc_feature 3865..3899
/note="5' UTR of amp, incl. RBS"
regulatory 3920..4201
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4158..4233
/note="SV40 ori"
CDS complement(4266..5126)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87917.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5828..6154
/note="pMB1 ori"
BASE COUNT 1609 a 1531 c 1488 g 1532 t
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt ccggactcag atcatcaaca agtttgtaca aaaaagctga acgagaaacg
1381 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
1441 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
1501 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg gcgagatttt
1561 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
1621 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
1681 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
1741 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
1801 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
1861 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
1921 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
1981 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
2041 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
2101 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
2161 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
2221 agtggcaggg cggggcgtaa agatctggat ccggcttact aaaagccaga taacagtatg
2281 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
2341 tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
2401 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
2461 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
2521 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag ggactggtga
2581 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
2641 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
2701 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
2761 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
2821 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
2881 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
2941 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
3001 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gttgatgatc
3061 caccggatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc
3121 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
3181 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
3241 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
3301 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
3361 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
3421 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
3481 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
3541 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
3601 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
3661 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
3721 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
3781 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
3841 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
3901 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
3961 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
4021 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
4081 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
4141 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
4201 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
4261 tcgagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
4321 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
4381 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
4441 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
4501 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
4561 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
4621 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
4681 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
4741 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
4801 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
4861 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
4921 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
4981 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
5041 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
5101 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
5161 cagggttatt gtcccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
5221 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
5281 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
5341 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
5401 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
5461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
5521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
5581 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
5641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
5701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
5761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
5821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
5881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
5941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
6001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
6061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
6121 atcccctgat tctgtggata accgtattac cgccatgcat
//