LOCUS LT727253 6038 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian vector pDEST-Etag, complete sequence. ACCESSION LT727253 VERSION LT727253.1 KEYWORDS . SOURCE Mammalian vector pDEST-Etag ORGANISM Mammalian vector pDEST-Etag other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6038) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6038 /organism="Mammalian vector pDEST-Etag" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4540." /db_xref="taxon:1945176" regulatory complement(1..17) /note="T7 promoter" /regulatory_class="promoter" regulatory complement(82..178) /note="SV40 polyA early" /regulatory_class="terminator" regulatory 82..178 /note="SV40 polyA late" /regulatory_class="terminator" regulatory complement(271..287) /note="T3 promoter" /regulatory_class="promoter" regulatory complement(351..407) /note="lacUV5 promoter" /regulatory_class="promoter" rep_origin complement(526..928) /note="pMB1 ori" CDS complement(1469..2329) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87890.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin complement(2460..2915) /note="f1 ori" regulatory 2989..4001 /note="sCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory 4008..4024 /note="SP6 promoter" /regulatory_class="promoter" misc_feature 4073..4111 /note="E-tag" regulatory complement(4142..4266) /note="attR1" /regulatory_class="attenuator" CDS 4543..5202 /transl_table=11 /note="chloramphenicol resistance" /protein_id="SJL87891.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" CDS 5544..5849 /transl_table=11 /note="ccdB" /protein_id="SJL87892.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" regulatory 5890..6014 /note="attR2" /regulatory_class="attenuator" BASE COUNT 1589 a 1407 c 1470 g 1572 t ORIGIN 1 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa 61 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 121 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 181 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg 241 cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt 301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 901 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 1021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 1081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 1141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 1201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 1261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 1321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 1381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 1441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 1501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 1561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 1621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 1681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 1741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 1801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 1861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 1921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 1981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 2041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 2101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 2161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 2221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 2281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 2341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 2401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta 2461 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 2521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 2581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 2641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 2701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 2761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 2821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 2881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa 2941 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga ccatagccaa 3001 ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa 3061 ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa 3121 ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacttggc actgtgccaa 3181 ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca 3241 agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag 3301 gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc 3361 aatactatgt attggcccta tgccatatag tattccatat atgggttttc ctattgacgt 3421 agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc 3481 atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat 3541 atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg 3601 cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga 3661 tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat 3721 ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg 3781 gaaggacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg 3841 cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg 3901 cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc 3961 tcgtttaggg aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac 4021 tatagaatac aagctacttg ttctttttgc aggatcccat cgatccccca tgggtgcgcc 4081 ggtgccgtat ccggacccgc tggaaccgcg tgcggcccat cgattcgaat tcaaggatca 4141 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 4201 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 4261 actatggcgg ccgcgggtga tgctgccaac ttagcggccg ctaagttggc agcatcaccc 4321 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat 4381 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt 4441 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta 4501 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact 4561 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag 4621 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag 4681 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg 4741 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat 4801 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg 4861 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt 4921 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca 4981 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc 5041 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg 5101 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat 5161 gaattacaac agtactgcga tgagtggcag ggcggggcgt aatctagagg atccggctta 5221 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat 5281 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag 5341 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg 5401 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc 5461 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc 5521 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc 5581 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg 5641 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg 5701 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg 5761 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg 5821 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg 5881 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta 5941 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc 6001 ttgtacaaag tggttcgatc ctctcgagcc tctagaac //