LOCUS LT727233 5990 bp DNA circular SYN 06-FEB-2017 DEFINITION Reporter vector p1168hIL6mC/EBP-luc+, complete sequence. ACCESSION LT727233 VERSION LT727233.1 KEYWORDS . SOURCE Reporter vector p1168hIL6mC/EBP-luc+ ORGANISM Reporter vector p1168hIL6mC/EBP-luc+ other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5990) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5990 /organism="Reporter vector p1168hIL6mC/EBP-luc+" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4498." /db_xref="taxon:1945188" misc_feature 32..672 /note="5' FR of hIL6" regulatory 673..1208 /note="hIL6 promoter mutant" /regulatory_class="promoter" CDS 1256..2908 /transl_table=11 /note="LUCm" /protein_id="SJL87828.1" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" regulatory 2996..3092 /note="SV40 polyA late" /regulatory_class="terminator" regulatory complement(2996..3092) /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(3309..3711) /note="pMB1 ori" CDS complement(4252..5112) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87829.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5244..5699 /note="f1 ori" regulatory complement(5830..5983) /note="synthetic polyA" /regulatory_class="terminator" BASE COUNT 1588 a 1430 c 1434 g 1538 t ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccgg ggatcctcct gcaagagaca ccatcctgag 61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag 121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt 181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg 241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac 301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag 361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac 421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg 481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc 541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg 601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag 661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga 721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg 781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa 841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac 901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg 961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc 1021 tagttgtgtc ttgcgatgct aaaggacgtc acagatatca atcttaataa ggtttccaat 1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggat 1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc 1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaag ccaccatgga 1261 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc 1321 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt 1381 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg 1441 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag 1501 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt 1561 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca 1621 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa 1681 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca 1741 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt 1801 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac 1861 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc 1921 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc 1981 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt 2041 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat 2101 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga 2161 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga 2221 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct 2281 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt 2341 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct 2401 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt 2461 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga 2521 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat 2581 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc 2641 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc 2701 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt 2761 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt 2821 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc 2881 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc 2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc 3181 gatcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 3241 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg 3301 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 3361 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 3421 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 3481 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 3541 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 3601 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 3661 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 3721 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 3781 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 3841 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 3901 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 3961 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 4021 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 4081 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 4141 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 4201 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 4261 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 4321 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 4381 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 4441 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 4501 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 4561 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 4621 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 4681 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 4741 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 4801 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 4861 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 4921 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 4981 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 5041 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 5101 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 5161 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 5221 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 5281 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 5341 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 5401 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 5461 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 5521 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 5581 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 5641 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 5701 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 5761 attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg 5821 agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa 5881 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa 5941 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata //