LOCUS LT727095 5338 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-E-hABIN-1-MAD, complete sequence. ACCESSION LT727095 VERSION LT727095.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-E-hABIN-1-MAD ORGANISM Mammalian expression vector pCAGGS-E-hABIN-1-MAD other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5338) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5338 /organism="Mammalian expression vector pCAGGS-E-hABIN-1-MAD" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5131." /db_xref="taxon:1944770" misc_feature 11..49 /note="E-tag" CDS <59..>532 /codon_start=1 /transl_table=11 /note="hABIN-1-MAD" /protein_id="SJL87415.1" /translation="TPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEEDFQ RERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKASG ERYHVEPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPPMAMEHPPPLPNS" regulatory 623..714 /note="3' UTR of rabbit beta-globin, incl. polyA" /regulatory_class="terminator" misc_feature 715..1071 /note="3' flanking region of rabbit beta-globin" regulatory complement(1149..1231) /note="lac promoter" /regulatory_class="promoter" rep_origin 1373..1448 /note="SV40 ori" regulatory 1479..1553 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(1479..1553) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(1668..2069) /note="pMB1 ori" CDS complement(2610..3470) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87416.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3606..3983 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 3989..5330 /note="AG promoter" /regulatory_class="promoter" misc_feature 4358..5282 /note="intron AG promoter" BASE COUNT 1144 a 1441 c 1491 g 1262 t ORIGIN 1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgccac 61 cccgccatca tctccaccaa cagcatttgg gagcccagaa ggagcagggg ccctcctaag 121 gaaacaggag ctggtcacgc agaatgagtt gctgaaacag caggtgaaga tcttcgagga 181 ggacttccag agggagcgca gtgatcgtga gcgcatgaat gaggagaagg aagagctgaa 241 gaagcaagtg gagaagctgc aggcccaggt caccctgtca aatgcccagc taaaagcatt 301 caaagatgag gagaaggcaa gagaagccct cagacagcag aagaggaaag caaaggcctc 361 aggagagcgt taccatgtgg agccccaccc agaacatctc tgcggggcct acccctacgc 421 ctacccgccc atgccagcca tggtgccaca ccatggcttc gaggactggt cccagatccg 481 ctacccccct ccccccatgg ccatggagca cccgccccca ctccccaact cgtgactcga 541 ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc 601 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat 661 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 721 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa 781 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat 841 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc 901 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt 961 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct 1021 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct 1081 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 1141 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg 1201 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 1261 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 1321 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 1381 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 1441 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata 1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 1561 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca 1621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 1681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 1741 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca 1801 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 1861 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 1921 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 1981 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 2041 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 2101 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 2161 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 2221 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 2281 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 2341 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 2401 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 2461 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 2521 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 2581 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 2641 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 2701 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 2761 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 2821 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 2881 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 2941 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 3001 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 3061 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 3121 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 3181 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 3241 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 3301 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 3361 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 3421 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 3481 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 3541 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 3601 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 3661 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 3721 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 3781 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 3841 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 3901 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 3961 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc 4021 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 4081 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg 4141 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 4201 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 4261 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg 4321 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 4381 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 4441 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 4501 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 4561 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 4621 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 4681 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 4741 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 4801 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 4861 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg 4921 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga 4981 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc 5041 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 5101 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct 5161 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 5221 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 5281 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgaa //