LOCUS LT727057 5912 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-crmA, complete sequence.
ACCESSION LT727057
VERSION LT727057.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-crmA
ORGANISM Mammalian expression vector pCAGGS-E-crmA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5912)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5912
/organism="Mammalian expression vector pCAGGS-E-crmA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4888."
/db_xref="taxon:1944761"
misc_feature 16..54
/note="E-tag"
CDS <64..1086
/codon_start=1
/transl_table=11
/note="cowpox virus crmA"
/protein_id="SJL87310.1"
/translation="DIFREIASSMKGENVFISPPSISSVLTILYYGANGSTAEQLSKY
VEKEADKNKDDISFKSMNKVYGRYSAVFKDSFLRKIGDNFQTVDFTDCRTVDAINKCV
DIFTEGKINPLLDEPLSPDTCLLAISAVYFKAKWLMPFEKEFTSDYPFYVSPTEMVDV
SMMSMYGEAFNHASVKESFGNFSIIELPYVGDTSMVVILPDNIDGLESIEQNLTDTNF
KKWCDSMDAMFIDVHIPKFKVTGSYNLVDALVKLGLTEVFGSTGDYSNMCNSDVSVDA
MIHKTYIDVNEEYTEAAAATCALVADCASTVTNEFCADHPFIYVIRHVDGKILFVGRY
CSPTTN"
regulatory 1202..1293
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1294..1650
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1728..1810)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1952..2027
/note="SV40 ori"
regulatory 2058..2132
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2058..2132)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2247..2648)
/note="pMB1 ori"
CDS complement(3189..4049)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87311.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4185..4562
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4568..5909
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4937..5861
/note="intron AG promoter"
BASE COUNT 1339 a 1476 c 1587 g 1510 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctgatatct tcagggaaat cgcatcttct atgaaaggag agaatgtatt catttctcca
121 ccgtcaatct cgtcagtatt gacaatactg tattatggag ctaatggatc cactgctgaa
181 cagctatcaa aatatgtaga aaaggaggcg gacaagaata aggatgatat ctcattcaag
241 tccatgaata aagtatatgg gcgatattct gcagtgttta aagattcctt tttgagaaaa
301 attggagata atttccaaac tgttgacttc actgattgtc gcactgtaga tgcgatcaac
361 aagtgtgttg atatcttcac tgaggggaaa attaatccac tattggatga accattgtct
421 ccagatacct gtctcctagc aattagtgcc gtatacttta aagcaaaatg gttgatgcca
481 tttgaaaagg aatttaccag tgattatccc ttttacgtat ctccaacgga aatggtagat
541 gtaagtatga tgtctatgta cggcgaggca tttaatcacg catctgtaaa agaatcattc
601 ggcaactttt caatcataga actgccatat gttggagata ctagtatggt ggtaattctt
661 ccagacaata ttgatggact agaatccata gaacaaaatc taacagatac aaattttaag
721 aaatggtgtg actctatgga tgctatgttt atcgatgtgc acattcccaa gtttaaggta
781 acaggctcgt ataatctggt ggatgcgcta gtaaagttgg gactgacaga ggtgttcggt
841 tcaactggag attatagcaa tatgtgtaat tcagatgtga gtgtcgacgc tatgatccac
901 aaaacgtata tagatgtcaa tgaagagtat acagaagcag ctgcagcaac ttgtgcgctg
961 gtggcagact gtgcatcaac agttacaaat gagttctgtg cagatcatcc gttcatctat
1021 gtgattaggc atgtcgatgg caaaattctt ttcgttggta gatattgctc tccaacaact
1081 aattaacccg ggacattaaa cgcgtgggta ccggatccgg caattcactc ctcaggtgca
1141 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
1201 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
1261 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
1321 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
1381 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
1441 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
1501 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
1561 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
1621 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
1681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
1741 gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat
1801 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
1861 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
1921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
1981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
2041 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
2101 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
2161 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
2221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
2281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
2341 cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
2401 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
2461 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
2521 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
2581 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
2641 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
2701 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
2761 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
2821 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
2881 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
2941 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
3001 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
3061 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
3121 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
3181 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
3241 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
3301 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
3361 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
3421 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
3481 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
3541 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
3601 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
3661 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
3721 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
3781 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
3841 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
3901 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
3961 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
4021 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
4081 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
4141 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac
4201 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
4261 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
4321 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
4381 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
4441 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
4501 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
4561 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
4621 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
4681 gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa
4741 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
4801 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg
4861 ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
4921 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
4981 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
5041 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
5101 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
5161 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
5221 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
5281 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
5341 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
5401 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
5461 ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
5521 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
5581 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
5641 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
5701 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
5761 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
5821 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
5881 ttattgtgct gtctcatcat tttggcaaag aa
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