LOCUS LT726878 5208 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(469-601), complete
sequence.
ACCESSION LT726878
VERSION LT726878.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(469-601)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(469-601)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5208)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5208
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(469-601)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5374."
/db_xref="taxon:1944800"
misc_feature 16..54
/note="E-tag"
CDS 64..465
/transl_table=11
/note="mABIN-1(469-601)"
/protein_id="SJL86752.1"
/translation="MTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQA
QVTLTNAQLKTLKEEEKAKEALKQQKRKAKASGERYHMEPHPEHVCGAYPYAYPPMPA
MVPHHAYKDWSQIRYPPPPVPMEHPPPHPNS"
regulatory 498..589
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 590..946
/note="3' FR of beta-globin"
regulatory complement(1024..1106)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1248..1323
/note="SV40 ori"
regulatory complement(1354..1428)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1354..1428
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1543..1944)
/note="pMB1 ori"
CDS complement(2485..3345)
/transl_table=11
/note="ampicillin"
/protein_id="SJL86753.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3481..3858
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3864..5205
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4233..5157
/note="intron AG promoter"
BASE COUNT 1123 a 1405 c 1440 g 1240 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagtgacac agaatgagtt gctgaaacag caggtaaaga tctttgaaga ggacttccag
121 agggaacgga gtgaccgtga acgcatgaat gaagagaagg aggagctgaa gaagcaagta
181 gagaagctgc aggcccaggt caccctgact aatgcccagc tcaaaactct caaagaggag
241 gagaaggcca aggaagccct caaacagcag aagaggaaag caaaggcttc gggagagcgc
301 taccacatgg aaccccaccc tgagcacgtc tgcggcgcct atccctatgc ctacccaccc
361 atgccagcca tggtacctca ccatgcctac aaggactggt cccagatccg ataccctcca
421 ccccctgtgc ccatggagca cccgccccca caccccaact cttgaccccc aatgccctgg
481 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
541 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
601 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
661 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
721 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
781 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
841 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
901 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
961 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
1021 attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc taatgagtga
1081 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
1141 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
1201 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
1261 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
1321 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
1381 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
1441 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
1501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
1561 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
1621 cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt gagcaaaagg
1681 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
1741 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
1801 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
1861 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
1921 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
1981 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
2041 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
2101 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
2161 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
2221 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
2281 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
2341 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
2401 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
2461 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
2521 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
2581 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
2641 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
2701 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
2761 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
2821 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
2881 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
2941 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
3001 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
3061 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
3121 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
3181 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
3241 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
3301 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
3361 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
3421 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
3481 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
3541 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
3601 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
3661 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
3721 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
3781 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
3841 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
3901 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
3961 tgggggcggg gggggggggg gggccccccc caggcggggc ggggcggggc gaggggcggg
4021 gcggggcgag gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
4081 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
4141 gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
4201 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
4261 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
4321 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
4381 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
4441 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
4501 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
4561 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
4621 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
4681 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
4741 ggccgcctcg ggccggggag ggctcggggg aaggggcgcg gcggcccccg gagcgccggc
4801 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
4861 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
4921 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
4981 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
5041 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
5101 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
5161 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaa
//