LOCUS LT726869 5371 bp DNA circular SYN 06-FEB-2017
DEFINITION Fungal expression vector pCSN43, complete sequence.
ACCESSION LT726869
VERSION LT726869.1
KEYWORDS .
SOURCE Fungal expression vector pCSN43
ORGANISM Fungal expression vector pCSN43
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5371)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5371
/organism="Fungal expression vector pCSN43"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8047."
/db_xref="taxon:1944724"
rep_origin complement(3..458)
/note="f1 ori"
regulatory 626..642
/note="T7 promoter"
/regulatory_class="promoter"
regulatory 674..1039
/note="trpC promoter"
/regulatory_class="promoter"
CDS 1040..2065
/transl_table=11
/note="hygromycin resistance"
/protein_id="SJL86723.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory 2235..2797
/note="trpC terminator"
/regulatory_class="terminator"
regulatory complement(3185..3201)
/note="T3 promoter"
/regulatory_class="promoter"
misc_feature complement(3227..3264)
/note="5' UTR of lacZ, incl. RBS"
regulatory complement(3265..3348)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(3440..3842)
/note="pMB1 ori"
CDS complement(4383..5243)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86724.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1299 a 1359 c 1400 g 1313 t
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
661 gccccccctc gaggtcgaca gaagatgata ttgaaggagc atttttgggc ttggctggag
721 ctagtggagg tcaacacatc aatgctattt tggtttagtc gtccaggcgg atcacaaaat
781 ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc acttgtaagc
841 agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat
901 catctgctgc ttggtgcacg ataacttgtg cgtttgtcaa gcaaggtaag tgaacgaccc
961 ggtcatacct tcttaagttc gcccttcctc cctttatttc agattcaatc tgacttacct
1021 attctaccga agcatcgata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
1081 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
1141 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
1201 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
1261 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
1321 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
1381 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
1441 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
1501 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
1561 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
1621 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
1681 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
1741 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
1801 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
1861 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
1921 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
1981 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
2041 cactcgtccg agggcaaagg aatagagtag atgccgaccg ggatccactt aacgttactg
2101 aaatcatcaa acagcttgac gaatctggat ataagatcgt tggtgtcgat gtcagctccg
2161 gagttgagac aaatggtgtt caggatctcg ataagatacg ttcatttgtc caagcagcaa
2221 agagtgcctt ctagtgattt aatagctcca tgtcaacaag aataaaacgc gttttcgggt
2281 ttacctcttc cagatacagc tcatctgcaa tgcattaatg cattgactgc aacctagtaa
2341 cgccttcagg ctccggcgaa gagaagaata gcttagcaga gctattttca ttttcgggag
2401 acgagatcaa gcagatcaac ggtcgtcaag agacctacga gactgaggaa tccgctcttg
2461 gctccacgcg actatatatt tgtctctaat tgtactttga catgctcctc ttctttactc
2521 tgatagcttg actatgaaaa ttccgtcacc agccctgggt tcgcaaagat aattgcatgt
2581 ttcttccttg aactctcaag cctacaggac acacattcat cgtaggtata aacctcgaaa
2641 tcattcctac taagatggta tacaatagta accatggttg cctagtgaat gctccgtaac
2701 acccaatacg ccggccgaaa cttttttaca actctcctat gagtcgttta cccagaatgc
2761 acaggtacac ttgtttagag gtaatccttc tttctagagg atcctctacg ccggacgcat
2821 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
2881 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
2941 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
3001 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
3061 gcataaggga gggacgcatc agcgtcgacg gtatcgataa gcttgatatc gaattcctgc
3121 agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
3181 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
3241 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
3301 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
3361 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
3421 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
3481 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
3541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
3601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
3661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
3721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
3781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
3841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
3901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
3961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
4021 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
4081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
4141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
4201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
4261 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
4321 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
4381 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
4441 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
4501 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
4561 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
4621 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
4681 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
4741 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
4801 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
4861 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
4921 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
4981 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
5041 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
5101 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
5161 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
5221 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
5281 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
5341 gttccgcgca catttccccg aaaagtgcca c
//