LOCUS LT726849 7354 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian vector pdcDNA-Myc, complete sequence. ACCESSION LT726849 VERSION LT726849.1 KEYWORDS . SOURCE Mammalian vector pdcDNA-Myc ORGANISM Mammalian vector pdcDNA-Myc other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7354) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7354 /organism="Mammalian vector pdcDNA-Myc" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 7212." /db_xref="taxon:1945173" regulatory 235..818 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory 863..879 /note="T7 promoter" /regulatory_class="promoter" regulatory 896..912 /note="SP6 promoter" /regulatory_class="promoter" misc_feature 964..993 /note="Myc epitope" misc_feature 1003..1032 /note="Myc epitope" misc_feature 1042..1071 /note="Myc epitope" misc_feature 1081..1110 /note="Myc epitope" misc_feature 1120..1149 /note="Myc epitope" misc_feature 1183..1212 /note="Myc epitope" regulatory complement(1240..1364) /note="attR1" /regulatory_class="attenuator" regulatory 1372..1424 /note="lacUV5 promoter" /regulatory_class="promoter" CDS 1473..2132 /transl_table=11 /note="chloramphenicol resistance" /protein_id="SJL86669.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" CDS 2474..2779 /transl_table=11 /note="ccdB" /protein_id="SJL86670.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" regulatory 2820..2944 /note="attR2" /regulatory_class="attenuator" regulatory complement(2981..2997) /note="SP6 promoter" /regulatory_class="promoter" regulatory 3023..3247 /note="BGH polyA" /regulatory_class="terminator" rep_origin 3293..3721 /note="f1 ori" regulatory 3726..3935 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 3892..3967 /note="SV40 ori" CDS 4059..4853 /transl_table=11 /note="Tn5 neomycin resistance" /protein_id="SJL86671.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 5027..5101 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(5027..5101) /note="SV40 polyA late" /regulatory_class="terminator" regulatory complement(5240..5323) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(5415..5817) /note="pMB1 ori" CDS complement(6358..7218) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86672.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1818 a 1780 c 1835 g 1921 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgattta 901 ggtgacacta tagaatacaa gctacttgtt ctttttgcag gatcccatcg atttaaagct 961 atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct 1021 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg 1081 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa 1141 gaggacttga atgaaatgga gagcttgggc gacctcacca tggagcaaaa gctcatttct 1201 gaagaggact tgaatttaga gatctcgaat tcaaggatca caagtttgta caaaaaagct 1261 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa 1321 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca 1381 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc 1441 cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca 1501 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc 1561 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga 1621 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc 1681 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc 1741 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc 1801 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa 1861 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct 1921 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg 1981 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc 2041 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac 2101 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca 2161 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt 2221 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga 2281 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc 2341 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag 2401 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac 2461 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct 2521 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct 2581 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat 2641 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat 2701 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct 2761 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc 2821 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct 2881 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag 2941 tggtgattcc cctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct 3001 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc 3061 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat 3121 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg 3181 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc 3241 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc 3301 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 3361 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 3421 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta 3481 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 3541 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 3601 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 3661 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 3721 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 3781 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 3841 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 3901 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 3961 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 4021 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 4081 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 4141 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 4201 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 4261 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 4321 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 4381 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 4441 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 4501 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 4561 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 4621 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 4681 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 4741 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 4801 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 4861 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 4921 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 4981 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 5161 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 5221 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 5281 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 5341 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 5401 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 5461 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 5521 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 5581 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 5641 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 5701 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 5761 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 5821 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 5881 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 5941 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 6001 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 6061 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 6121 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 6181 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 6241 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 6301 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 6361 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 6421 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 6481 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 6541 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 6601 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 6661 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 6721 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 6781 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 6841 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 6901 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 6961 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 7021 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 7081 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 7141 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 7201 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 7261 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 7321 gcgcacattt ccccgaaaag tgccacctga cgtc //