LOCUS LT726821 5981 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mCASP-1-4DE, complete
sequence.
ACCESSION LT726821
VERSION LT726821.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mCASP-1-4DE
ORGANISM Mammalian expression vector pCAGGS-E-mCASP-1-4DE
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5981)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5981
/organism="Mammalian expression vector
pCAGGS-E-mCASP-1-4DE"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6093."
/db_xref="taxon:1944807"
misc_feature 16..54
/note="E-tag"
CDS <64..1269
/codon_start=1
/transl_table=11
/note="mCASP-1 mutant"
/protein_id="SJL86591.1"
/translation="ADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKIKL
ANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAGILELQSAPSAETFVATEE
SKGGHPSSSETKEEQNKEEGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTRT
RLALIICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFAA
CPEHKTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPK
VIIIQACRGEKQGVVLLKESVRDSEEDFLTDAIFEDEGIKKAHIEKDFIAFCSSTPDN
VSWRHPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLT
KRFYLFPGH"
misc_feature 367..369
/note="D103E"
misc_feature 424..426
/note="D122E"
misc_feature 946..948
/note="D296E"
misc_feature 1000..1002
/note="D314E"
regulatory 1271..1362
/note="3' UTR of beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1363..1719
/note="3' flanking region rabbit beta-globin"
regulatory complement(1797..1879)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2021..2096
/note="SV40 ori"
regulatory 2127..2201
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2127..2201)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2316..2717)
/note="pMB1 ori"
CDS complement(3258..4118)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86592.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4254..4631
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4637..5978
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5006..5930
/note="intron AG promoter"
BASE COUNT 1383 a 1502 c 1606 g 1490 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctgctgaca agatcctgag ggcaaagagg aagcaattta tcaactcagt gagtataggg
121 acaataaatg gattgttgga tgaactttta gagaagagag tgctgaatca ggaagaaatg
181 gataaaataa aacttgcaaa cattactgct atggacaagg cacgggacct atgtgatcat
241 gtctctaaaa aagggcccca ggcaagccaa atctttatca cttacatttg taatgaagac
301 tgctacctgg caggaattct ggagcttcaa tcagctccat cagctgaaac atttgttgct
361 acagaagaat ctaaaggtgg ccatccttca tcctcagaaa caaaggaaga acagaacaaa
421 gaagagggta cctttccagg actgactggg accctcaagt tttgcccttt agaaaaagcc
481 cagaagttat ggaaagaaaa tccttcagag atttatccaa taatgaatac aaccactcgt
541 acacgtcttg ccctcattat ctgcaacaca gagtttcaac atctttctcc gagggttgga
601 gctcaagttg acctcagaga aatgaagttg ctgctggagg atctggggta taccgtgaaa
661 gtgaaagaaa atctcacagc tctggagatg gtgaaagagg tgaaagaatt tgctgcctgc
721 ccagagcaca agacttctga cagtactttc cttgtattca tgtctcatgg tatccaggag
781 ggaatatgtg ggaccacata ctctaatgaa gtttcagata ttttaaaggt tgacacaatc
841 tttcagatga tgaacacttt gaagtgccca agcttgaaag acaagcccaa ggtgatcatt
901 attcaggcat gccgtggaga gaaacaagga gtggtgttgt taaaagaatc agtaagagac
961 tctgaagagg atttcttaac ggatgcaatt tttgaagatg aaggcattaa gaaggcccat
1021 atagagaaag attttattgc tttctgctct tcaacaccag ataatgtgtc ttggagacat
1081 cctgtcaggg gctcactttt cattgagtca ctcatcaaac acatgaaaga atatgcctgg
1141 tcttgtgact tggaggacat tttcagaaag gttcgatttt catttgaaca accagaattt
1201 aggctacaga tgcccactgc tgatagggtg accctgacaa aacgtttcta cctcttcccg
1261 ggacattaaa gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
1321 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
1381 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
1441 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
1501 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
1561 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
1621 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
1681 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
1741 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
1801 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact
1861 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
1921 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
1981 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
2041 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
2101 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
2161 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
2221 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
2281 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
2341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
2401 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
2461 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
2521 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
2581 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
2641 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
2701 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
2761 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
2821 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
2881 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
2941 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
3001 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
3061 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
3121 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
3181 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
3241 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
3301 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
3361 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
3421 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
3481 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
3541 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
3601 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
3661 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
3721 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
3781 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
3841 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
3901 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
3961 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
4021 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
4081 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
4141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
4201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
4261 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
4321 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
4381 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
4441 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
4501 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
4561 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
4621 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
4681 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
4741 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
4801 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
4861 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt
4921 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
4981 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
5041 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
5101 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
5161 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
5221 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
5281 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
5341 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
5401 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
5461 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
5521 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc
5581 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
5641 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
5701 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
5761 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
5821 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
5881 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
5941 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a
//