LOCUS JO316084 286 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c54228_c mRNA sequence. ACCESSION JO316084 VERSION JO316084.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 286) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 286) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..286 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 59 a 55 c 38 g 134 t ORIGIN 1 aattaatctt caatcacgaa cgtcatgcac gttttgtcag atgttgttta tttcacatcg 61 gaatcgtcga gtattttttc cttctcttgt tcgtctcgcc attgtctata tgaaaatcaa 121 gcattgatgt ttttctctag ttgtatagtt ttcgttgttt ttcttcttct tctagcaata 181 ttcgacctat ttcgaaaatt ctctctcttt ttttcttcat ttgtttatac atatttgtcg 241 tgattctctt tctctcacat acacgtgttg ttttagttga atcaat //