LOCUS CR536518 1134 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A1120D for
gene ACTA2, actin, alpha 2, smooth muscle, aorta; complete cds,
incl. stopcodon.
ACCESSION CR536518
VERSION CR536518.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1134)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1134)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A1120D, ORFNo 3085
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A1120D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site:
(stop)GACCCAGCTTTCTT..att
Compared to the reference sequence NM_001613 (gi4501882)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1134
/db_xref="H-InvDB:HIT000268455"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A1120D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1134
/codon_start=1
/gene="ACTA2"
/db_xref="GOA:P62736"
/db_xref="H-InvDB:HIT000268455.13"
/db_xref="HGNC:HGNC:130"
/db_xref="InterPro:IPR004000"
/db_xref="InterPro:IPR004001"
/db_xref="InterPro:IPR020902"
/db_xref="UniProtKB/Swiss-Prot:P62736"
/protein_id="CAG38756.1"
/translation="MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQG
VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEE
HPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDG
VTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKL
CYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGI
HETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP
PERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF"
BASE COUNT 278 a 310 c 291 g 255 t
ORIGIN
1 atgtgtgaag aagaggacag cactgccttg gtgtgtgaca atggctctgg gctctgtaag
61 gccggctttg ctggggacga tgctcccagg gctgttttcc catccattgt gggacgtccc
121 agacatcagg gggtgatggt gggaatggga caaaaagaca gctacgtggg tgacgaagca
181 cagagcaaaa gaggaatcct gaccctgaag tacccgatag aacatggcat catcaccaac
241 tgggacgaca tggaaaagat ctggcaccac tctttctaca atgagcttcg tgttgcccct
301 gaagagcatc ccaccctgct cacggaggca cccctgaacc ccaaggccaa ccgggagaaa
361 atgactcaaa ttatgtttga gactttcaat gtcccagcca tgtatgtggc tatccaggcg
421 gtgctgtctc tctatgcctc tggacgcaca actggcatcg tgctggactc tggagatggt
481 gtcacccaca atgtccccat ctatgagggc tatgccttgc cccatgccat catgcgtctg
541 gatctggctg gccgagatct cactgactac ctcatgaaga tcctgactga gcgtggctat
601 tccttcgtta ctactgctga gcgtgagatt gtccgggaca tcaaggagaa actgtgttat
661 gtagctctgg actttgaaaa tgagatggcc actgccgcat cctcatcctc ccttgagaag
721 agttacgagt tgcctgatgg gcaagtgatc accatcggaa atgaacgttt ccgctgccca
781 gagaccctgt tccagccatc cttcatcggg atggagtctg ctggcatcca tgaaaccacc
841 tacaacagca tcatgaagtg tgatattgac atcaggaagg acctctatgc taacaatgtc
901 ctatcagggg gcaccactat gtaccctggc attgccgacc gaatgcagaa ggagatcacg
961 gccctagcac ccagcaccat gaagatcaag atcattgccc ctccggagcg caaatactct
1021 gtctggatcg gtggctccat cctggcctct ctgtccacct tccagcagat gtggatcagc
1081 aaacaggaat acgatgaagc cgggccttcc attgtccacc gcaaatgctt ctaa
//