LOCUS CR457336 867 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0613D for gene ASB8, ankyrin repeat and SOCS box-containing 8; complete cds, incl. stopcodon. ACCESSION CR457336 VERSION CR457336.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 867) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 867) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834E0613D, ORFNo 2413 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0613D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_024095 we found amino acid exchange(s) at position (first base of changed triplet): 862(glu->asp) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..867 /db_xref="H-InvDB:HIT000268186" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834E0613D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..867 /codon_start=1 /gene="ASB8" /db_xref="GOA:Q6IA19" /db_xref="H-InvDB:HIT000268186.13" /db_xref="InterPro:IPR001496" /db_xref="InterPro:IPR002110" /db_xref="InterPro:IPR020683" /db_xref="InterPro:IPR036036" /db_xref="InterPro:IPR036770" /db_xref="InterPro:IPR037332" /db_xref="UniProtKB/TrEMBL:Q6IA19" /protein_id="CAG33617.1" /translation="MSSSMWYIMQSIQSKYSLSERLIRTIAAIRSFPHDNVEDLIRGG ADVNCTHGTLKPLHCACMVSDADCVELLLEKGAEVNALDGYNRTALHYAAEKDEACVE VLLEYGANPNALDGNRDTPLHWAAFKNNAECVRALLESGASVNALDYNNDTPLSWAAM KGNLESVSILLDYGAEVRVINLIGQTPISRLVALLVRGLGTEKEDSCFELLHRAVGHF ELRKNGTMPREVARDPQLCEKLTVLCSAPGTLKTLARYAVRRSLGLQYLPDAVKGLPL PASLKEYLLLLD" BASE COUNT 219 a 215 c 229 g 204 t ORIGIN 1 atgagttcca gtatgtggta tattatgcag agcattcaga gcaaatactc tctctccgag 61 cgcttaatcc gaacaattgc tgccatccgt tccttcccac atgataatgt agaggacctc 121 atcagagggg gagcagatgt gaactgcact catggcacac tgaagccctt gcactgtgcc 181 tgtatggtgt cagatgctga ctgtgtggag ttacttctgg aaaaaggagc cgaggtgaat 241 gccctggatg ggtataaccg aacagccctc cactatgcag cagagaaaga tgaggcttgt 301 gtggaggtcc tattggagta tggtgcaaac cccaatgctt tggatggcaa cagagatacc 361 ccacttcact gggcagcctt taagaacaat gctgagtgtg tgcgggctct cctagagagc 421 ggggcctctg tcaatgccct ggattacaac aatgatacac cgctcagctg ggctgccatg 481 aagggaaatc ttgagagtgt cagcatcctt ctggattatg gcgcagaggt cagagtcatc 541 aacctaatag gccagacacc catctcccgc ctggtggctc tgctagtcag gggacttgga 601 acagagaaag aggactcttg ctttgagctc ctccacagag ctgttggaca ctttgaattg 661 aggaaaaatg gcaccatgcc acgagaggtg gccagagacc cgcagctatg tgaaaaactg 721 actgttctgt gctcagctcc aggaactcta aaaacactcg ctcgctatgc cgtgcgccgt 781 agcctgggac tccagtatct ccccgatgca gtgaagggcc ttccactgcc agcttctttg 841 aaggaatacc tgttactttt agattaa //