LOCUS CR457320 1533 bp mRNA linear HUM 21-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F0313D for gene TOE1, target of EGR1, member 1 (nuclear); complete cds, incl. stopcodon. ACCESSION CR457320 VERSION CR457320.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1533) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 1533) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834F0313D, ORFNo 2371 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F0313D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence BC009364 we found amino acid exchange(s) at position (first base of changed triplet): 34(ala->thr) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..1533 /db_xref="H-InvDB:HIT000268170" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834F0313D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..1533 /codon_start=1 /gene="TOE1" /db_xref="GOA:Q96GM8" /db_xref="H-InvDB:HIT000268170.13" /db_xref="HGNC:HGNC:15954" /db_xref="InterPro:IPR000571" /db_xref="InterPro:IPR006941" /db_xref="InterPro:IPR012337" /db_xref="InterPro:IPR036397" /db_xref="PDB:2FC6" /db_xref="UniProtKB/Swiss-Prot:Q96GM8" /protein_id="CAG33601.1" /translation="MAADSDDGAVSTPAASDGGVSKSTTSGEELVVQVPVVDVQSNNF KEMWPSLLLAIKTANFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSILSLGL ACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQFLIQHGFNFNQQYAQGIPYHK GNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADL CEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSS MRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAED KRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNL PHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYV MAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWG SS" BASE COUNT 380 a 412 c 416 g 325 t ORIGIN 1 atggccgccg acagtgacga tggcgcagtt tcaactcccg cagcttccga cggtggtgtc 61 agcaaaagca caacatctgg ggaggagcta gtagtccagg ttcccgtagt ggatgtgcaa 121 agcaacaact tcaaggagat gtggccatcc ctcctgctag ccataaagac agctaatttc 181 gtggctgtgg acacggagct gagtgggctt ggggacagga agagtttgct gaaccagtgc 241 attgaggaac gttacaaggc cgtgtgtcat gctgccagga cccgttctat cctttccctg 301 ggcctcgcct gcttcaagcg gcagccagac aagggtgaac attcctatct ggctcaagtg 361 ttcaatctca ctctgctgtg catggaggag tatgtcatag aaccaaagtc tgtgcagttc 421 ctgatacagc atggcttcaa cttcaaccag cagtatgccc aaggcatccc ctaccataag 481 ggcaatgaca agggtgatga gagccagagc cagtcagtac ggaccctatt cctggagcta 541 atccgagccc gccggcccct ggtgctacac aatggcctta tagacttggt gttcctgtac 601 cagaacttct atgcacacct ccctgagagt ctgggaacct tcaccgctga cctgtgtgag 661 atgttcccag caggcattta tgacaccaaa tatgctgctg agtttcatgc ccgtttcgtg 721 gcctcctact tagaatatgc cttccggaaa tgtgaacggg aaaatgggaa gcagcgggca 781 gctggcagcc cacaccttac cctggagttc tgcaactatc cttccagcat gagggaccat 841 attgattacc gctgctgcct gcccccagca acccaccgtc ctcatcccac cagcatctgt 901 gacaacttct cggcttatgg ctggtgcccc ctgggaccac agtgtcctca gtctcacgat 961 attgacctta tcattgacac tgatgaggct gcggcagagg acaagcggcg acggcgacga 1021 cgtagggaaa aacggaagag ggctttattg aacctaccgg ggacacagac ctctggggaa 1081 gctaaggatg gtcctcccaa gaagcaggtc tgtggggata gcatcaagcc tgaagaaacc 1141 gagcaggagg tggctgccga tgaaactagg aacctgcctc actccaagca aggcaacaaa 1201 aatgacttag agatggggat taaggcagca aggcctgaaa tagctgatag agctacctca 1261 gaagtgccag ggagccaagc cagtcctaac ccagtgcctg gggatggatt gcaccgggct 1321 ggttttgatg cctttatgac aggttatgtg atggcctatg tggaagtgag ccagggaccg 1381 cagccctgca gctctggacc ctggctccct gaatgccaca ataaggtata tttgagtggc 1441 aaagctgtac ccctcacagt ggccaagagc cagttctctc gttcctccaa agcccacaat 1501 cagaagatga agctcacttg gggcagtagt taa //