LOCUS CR450306 1209 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E031D for
gene PTEN, phosphatase and tensin homolog (mutated in multiple
advanced cancers 1); complete cds; without stopcodon.
ACCESSION CR450306
VERSION CR450306.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1209)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1209)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E031D, ORFNo 127
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E031D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence BC005821 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1209
/db_xref="H-InvDB:HIT000267215"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E031D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1209
/codon_start=1
/gene="PTEN"
/db_xref="GOA:P60484"
/db_xref="H-InvDB:HIT000267215.12"
/db_xref="HGNC:HGNC:9588"
/db_xref="InterPro:IPR000340"
/db_xref="InterPro:IPR003595"
/db_xref="InterPro:IPR014020"
/db_xref="InterPro:IPR016130"
/db_xref="InterPro:IPR017361"
/db_xref="InterPro:IPR029021"
/db_xref="InterPro:IPR029023"
/db_xref="PDB:1D5R"
/db_xref="PDB:2KYL"
/db_xref="PDB:4O1V"
/db_xref="PDB:5BUG"
/db_xref="PDB:5BZX"
/db_xref="PDB:5BZZ"
/db_xref="UniProtKB/Swiss-Prot:P60484"
/protein_id="CAG29302.1"
/translation="MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEG
VYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIK
PFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRT
RDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVC
QLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVN
TFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKAN
RYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPF
DEDQHTQITKV"
BASE COUNT 427 a 223 c 243 g 316 t
ORIGIN
1 atgacagcca tcatcaaaga gatcgttagc agaaacaaaa ggagatatca agaggatgga
61 ttcgacttag acttgaccta tatttatcca aacattattg ctatgggatt tcctgcagaa
121 agacttgaag gcgtatacag gaacaatatt gatgatgtag taaggttttt ggattcaaag
181 cataaaaacc attacaagat atacaatctt tgtgctgaaa gacattatga caccgccaaa
241 tttaattgca gagttgcaca atatcctttt gaagaccata acccaccaca gctagaactt
301 atcaaaccct tttgtgaaga tcttgaccaa tggctaagtg aagatgacaa tcatgttgca
361 gcaattcact gtaaagctgg aaagggacga actggtgtaa tgatatgtgc atatttatta
421 catcggggca aatttttaaa ggcacaagag gccctagatt tctatgggga agtaaggacc
481 agagacaaaa agggagtaac tattcccagt cagaggcgct atgtgtatta ttatagctac
541 ctgttaaaga atcatctgga ttatagacca gtggcactgt tgtttcacaa gatgatgttt
601 gaaactattc caatgttcag tggcggaact tgcaatcctc agtttgtggt ctgccagcta
661 aaggtgaaga tatattcctc caattcagga cccacacgac gggaagacaa gttcatgtac
721 tttgagttcc ctcagccgtt acctgtgtgt ggtgatatca aagtagagtt cttccacaaa
781 cagaacaaga tgctaaaaaa ggacaaaatg tttcactttt gggtaaatac attcttcata
841 ccaggaccag aggaaacctc agaaaaagta gaaaatggaa gtctatgtga tcaagaaatc
901 gatagcattt gcagtataga gcgtgcagat aatgacaagg aatatctagt acttacttta
961 acaaaaaatg atcttgacaa agcaaataaa gacaaagcca accgatactt ttctccaaat
1021 tttaaggtga agctgtactt cacaaaaaca gtagaggagc cgtcaaatcc agaggctagc
1081 agttcaactt ctgtaacacc agatgttagt gacaacgaac ctgatcatta tagatattct
1141 gacaccactg actctgatcc agagaatgaa ccttttgatg aagatcagca tacacaaatt
1201 acaaaagtc
//