LOCUS       CN794415                 514 bp    mRNA    linear   EST 08-FEB-2011
DEFINITION  JA001P03P104 MYCELIUM Dactylellina haptotyla cDNA clone
            JA001P03P104, mRNA sequence.
ACCESSION   CN794415
VERSION     CN794415.1
DBLINK      BioSample: SAMN00175015
KEYWORDS    EST.
SOURCE      Dactylellina haptotyla (Monacrosporium haptotylum)
  ORGANISM  Dactylellina haptotyla
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Orbiliomycetes; Orbiliales; Orbiliaceae; Dactylellina.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Ahren,D., Tholander,M., Fekete,C., Rajashekar,B., Friman,E.,
            Johansson,T. and Tunlid,A.
  TITLE     Comparison of gene expression in trap cells and vegetative hyphae
            of the nematophagous fungus Monacrosporium haptotylum
  JOURNAL   Microbiology (Reading, Engl.) 151 (3), 789-803 (2005)
   PUBMED   15758225
COMMENT     Contact: Balaji Rajashekar, Anders Tunlid
            Fungal-Host Interaction Group (FHIG)
            Microbial Ecology, Institution of Ecology, Lund University
            Ecology Building, Lund University, SE-223 62 Lund, Sweden
            Tel: +46-46-222 37 63
            Fax: +46-46-222 41 58
            Email: Balaji.Rajashekar@mbioekol.lu.se ,
            Anders.Tunlid@mbioekol.lu.se
            PCR PRimers
            FORWARD: P104 (5'-GGGAAGCGCGCCATTGTGTT-3')
            BACKWARD: P105 (5'-AGTGAGCTCGAATTGCGGCC-3')
            Seq primer: P104.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Dactylellina haptotyla"
                     /mol_type="mRNA"
                     /strain="CBS 200.50"
                     /db_xref="taxon:430498"
                     /clone="JA001P03P104"
                     /tissue_type="Vegetative mycelium"
                     /clone_lib="SAMN00175015 MYCELIUM"
                     /dev_stage="Saprophytic"
                     /lab_host="Escherichia coli XL1-Blue"
                     /note="Vector: pTriplEx2; Site_1: SfiI; Site_2: SfiI; This
                     EST clone is originating from one of four cDNA libraries.
                     These libraries were constructed for transcript profiling
                     of nematophagous ascomycete Monacrosporium haptotylum in
                     interaction with Caenorhabditis elegans. The library
                     'MYCELIUM' is constructed from vegetative mycelium in a
                     saprophytic stage, the second library 'KNOB' is
                     constructed from the separated infection structure and
                     finally the '4h-POST INFECTION' and the '24h-POST
                     INFECTION' libraries represent early (4h) and late (24h)
                     post-infection stages. These libraries where analayzed in
                     parallel and 1729 (MYCELIUM), 1450 (KNOB), 1195 (4h-POST
                     INFECTION) and 2741 (24h-POST INFECTION) high-quality
                     (PHRED 20) ESTs of >99bp have been deposited. The cDNA
                     libraries were constructed from total RNA using the SMART
                     cDNA library Construction kit (#K1051-1, Clontech, Palo
                     Alto, CA, USA) according to the manufacturer's
                     instructions. Full-length cDNAs were trimmed by SfiI,
                     fractionated and directionally ligated into
                     (lambda)pTriplEx2 arms. The lambda library was converted
                     to a plasmid library via site-specific recombination at
                     loxP sites in a Cre+ strain (E. coli XL1-Blue). Plasmid
                     clones were randomly collected and analysed by DNA
                     sequencing using a plasmid-specific forward primer
                     (P104)."
BASE COUNT          179 a          118 c          102 g          115 t
ORIGIN      
        1 ggggactctc atcgagatat tacaacgcgc acagcgaaaa ctaccaaaga ttattttatt
       61 ctactatttg tattcgaaga ttcgataaat caaagaagag ggaaaaatga cataccctga
      121 gggcacccac gttaccttca ctaactcctc aggtggcgag gaagtcggca tcgtccagag
      181 ctacaacgat ggcacctact ctatccaagt caagtccaaa tccatcgtct ctgtcagcga
      241 gggcagcgtc aaggagctca aggtctcggc cacccaaaag gacaactgcc cagtcgttgg
      301 aaccgcctaa acagctggcg ggatcaaaat caaggaattt aacagttcgg gaggcccttt
      361 taaaaatcgt ttgtcaaccc aggatatatt ttaaaagtag gcaattaagt atattttgca
      421 cctgcatcct agttgcagcg tttcgtataa gacatcaaat atccaacgct ttgaactcct
      481 tgaacgaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//