LOCUS CN794415 514 bp mRNA linear EST 08-FEB-2011 DEFINITION JA001P03P104 MYCELIUM Dactylellina haptotyla cDNA clone JA001P03P104, mRNA sequence. ACCESSION CN794415 VERSION CN794415.1 DBLINK BioSample: SAMN00175015 KEYWORDS EST. SOURCE Dactylellina haptotyla (Monacrosporium haptotylum) ORGANISM Dactylellina haptotyla Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Orbiliomycetes; Orbiliales; Orbiliaceae; Dactylellina. REFERENCE 1 (bases 1 to 514) AUTHORS Ahren,D., Tholander,M., Fekete,C., Rajashekar,B., Friman,E., Johansson,T. and Tunlid,A. TITLE Comparison of gene expression in trap cells and vegetative hyphae of the nematophagous fungus Monacrosporium haptotylum JOURNAL Microbiology (Reading, Engl.) 151 (3), 789-803 (2005) PUBMED 15758225 COMMENT Contact: Balaji Rajashekar, Anders Tunlid Fungal-Host Interaction Group (FHIG) Microbial Ecology, Institution of Ecology, Lund University Ecology Building, Lund University, SE-223 62 Lund, Sweden Tel: +46-46-222 37 63 Fax: +46-46-222 41 58 Email: Balaji.Rajashekar@mbioekol.lu.se , Anders.Tunlid@mbioekol.lu.se PCR PRimers FORWARD: P104 (5'-GGGAAGCGCGCCATTGTGTT-3') BACKWARD: P105 (5'-AGTGAGCTCGAATTGCGGCC-3') Seq primer: P104. FEATURES Location/Qualifiers source 1..514 /organism="Dactylellina haptotyla" /mol_type="mRNA" /strain="CBS 200.50" /db_xref="taxon:430498" /clone="JA001P03P104" /tissue_type="Vegetative mycelium" /clone_lib="SAMN00175015 MYCELIUM" /dev_stage="Saprophytic" /lab_host="Escherichia coli XL1-Blue" /note="Vector: pTriplEx2; Site_1: SfiI; Site_2: SfiI; This EST clone is originating from one of four cDNA libraries. These libraries were constructed for transcript profiling of nematophagous ascomycete Monacrosporium haptotylum in interaction with Caenorhabditis elegans. The library 'MYCELIUM' is constructed from vegetative mycelium in a saprophytic stage, the second library 'KNOB' is constructed from the separated infection structure and finally the '4h-POST INFECTION' and the '24h-POST INFECTION' libraries represent early (4h) and late (24h) post-infection stages. These libraries where analayzed in parallel and 1729 (MYCELIUM), 1450 (KNOB), 1195 (4h-POST INFECTION) and 2741 (24h-POST INFECTION) high-quality (PHRED 20) ESTs of >99bp have been deposited. The cDNA libraries were constructed from total RNA using the SMART cDNA library Construction kit (#K1051-1, Clontech, Palo Alto, CA, USA) according to the manufacturer's instructions. Full-length cDNAs were trimmed by SfiI, fractionated and directionally ligated into (lambda)pTriplEx2 arms. The lambda library was converted to a plasmid library via site-specific recombination at loxP sites in a Cre+ strain (E. coli XL1-Blue). Plasmid clones were randomly collected and analysed by DNA sequencing using a plasmid-specific forward primer (P104)." BASE COUNT 179 a 118 c 102 g 115 t ORIGIN 1 ggggactctc atcgagatat tacaacgcgc acagcgaaaa ctaccaaaga ttattttatt 61 ctactatttg tattcgaaga ttcgataaat caaagaagag ggaaaaatga cataccctga 121 gggcacccac gttaccttca ctaactcctc aggtggcgag gaagtcggca tcgtccagag 181 ctacaacgat ggcacctact ctatccaagt caagtccaaa tccatcgtct ctgtcagcga 241 gggcagcgtc aaggagctca aggtctcggc cacccaaaag gacaactgcc cagtcgttgg 301 aaccgcctaa acagctggcg ggatcaaaat caaggaattt aacagttcgg gaggcccttt 361 taaaaatcgt ttgtcaaccc aggatatatt ttaaaagtag gcaattaagt atattttgca 421 cctgcatcct agttgcagcg tttcgtataa gacatcaaat atccaacgct ttgaactcct 481 tgaacgaaaa aaaaaaaaaa aaaaaaaaaa aaaa //