LOCUS CK335105 609 bp mRNA linear EST 12-MAY-2010 DEFINITION H3141H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3141H10 5', mRNA sequence. ACCESSION CK335105 VERSION CK335105.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 609) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3141 row: H column: 10 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..609 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3141H10-5" /db_xref="taxon:10090" /clone="H3141H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 166 a 142 c 161 g 140 t ORIGIN 1 ggatgctggt agagaagatg ggtcgagagg ccgtggagct ggggcatggg gaggtgaaca 61 tcaccggtgt ggaagagaac actttgattg ccagcctctg tgaccttctg gagaggatct 121 ggagccatgg gctacaagtg aagcagggga aatcagcttt gtggtcccat ctgttacatt 181 atcaggaaaa ccggcagaga aaactcacat caggaagcct tagtacctca ggaatacttc 241 ttgattcaga acggagaaag tctgatgcca gtgccgtcat gtctcccctg aggatttccc 301 taattcagga tatgaggcac atccagaata ttggggaaat caagactgat gtgggaaaag 361 ccagagcatg ggtgcgactg tccatggaga agaaattact gtccagacac ctgaagcagc 421 ttctttcaga ccatgagctc accaagaagc tgtacaagcg ttacgccttc ctgcgctgtg 481 atgacgagaa ggagcagttc ctctatcacc tgctctcctt caacgcagtg gactacttct 541 gcttcaccaa cgtctttaca accatcctga ttccatacca cattttgatt gtaccaagca 601 agaagctgg //