LOCUS       CK334985                 546 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3129D12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3129D12 5', mRNA sequence.
ACCESSION   CK334985
VERSION     CK334985.1
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: H3129  row: D  column: 12
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3129D12-5"
                     /db_xref="taxon:10090"
                     /clone="H3129D12"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          124 a          145 c          147 g          130 t
ORIGIN      
        1 aatcaccatg gcggctggga ccctgtacac ataccctgaa aattggaggg ccttcaaggc
       61 cctcatcgct gctcagtaca gcggggctca ggtccgcgtg ctctccgcac caccccactt
      121 ccactttggc caaaccaacc gcacccctgg atttctccga aaatttcctg ccggcaaggt
      181 tccagcattt gagggtgatg atggattctg tgtgtttgag agcaatgcca ttgcctatta
      241 tgtaagcaat gaggagctgc gaggaagtac gccagaggcg gcagctcagg tggtgcagtg
      301 ggtcagcttt gctgacagtg acatcgttcc tccagctagt acctgggtgt tccccacctt
      361 gggcattatg caccacaaca aacaggccac tgaaaatgcg aaagaggagg tgaagcgaat
      421 cctggggctg ctggacactc acttgaagac tcgaactttt ctagtgggcg agcgagtgac
      481 actggctgat attacagttg tctgtaccct gttgtggctc tataaacagg tcttagagcc
      541 ttcttt
//