LOCUS       CK334446                 549 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3030E11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3030E11 5', mRNA sequence.
VERSION     CK334446.1
DBLINK      BioSample: LIBEST_008622
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Plate: H3030  row: E  column: 11
            Seq primer: M13 Reverse
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Mus musculus"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          127 a          173 c          123 g          126 t
        1 ggaacaacct gagtgaggtg gaacaagcct ggatagccaa cggcagtgtt ggggagcccg
       61 tgatcaagtg tgagttcgag aaggttatca gcatggagta catggtctac ttcaacttct
      121 tcgtctgggt gctgccgcca ttgctcctca tggttcttat ctacctggag gtcttctacc
      181 tgatccgcaa gcagctcaac aaaaaggtgt cagcctcctc tggtgacccc cagaagtact
      241 acgggaagga gctcaagatc gccaagtcgc tggccctcat cctcttcctc tttgccctca
      301 gctggctacc gctacacatc ttgaactgca tcaccctctt ctgccccacc tgccagaaac
      361 ccagcatcct catctacatt gccatcttcc tcacacacgg caactcggcc atgaacccca
      421 tcgtctatgc cttccgaatc cacaagttcc gggtcacctt tctgaagatt tggaacgacc
      481 acttccgatg ccagcccaag ccccccattg aggaagatat cccagaggag aaagctgatg
      541 actaggctc