LOCUS CK334118 574 bp mRNA linear EST 12-MAY-2010 DEFINITION H3006B10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3006B10 5', mRNA sequence. ACCESSION CK334118 VERSION CK334118.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 574) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3006 row: B column: 10 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..574 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3006B10-5" /db_xref="taxon:10090" /clone="H3006B10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 130 a 161 c 165 g 118 t ORIGIN 1 ggaggctaac gggcctgccg atggctacgc tgcgattgcc caggcggaca ggttgactca 61 ggagcccgag agcatccgga agtggagaga ggagcagaag aaaaggctgc aggagttaga 121 cgctgcctcg aaggtgaccg aacaggagtg gcgggagaag gccaagaaag acctggagga 181 gtggaaccag cgccaaagtg aacaggttga gaaggccaag caaacaccac tgtcccgcta 241 ggtgcccgtc acgagaacgg ccgcgaagca catgggcctt gctggggcag aggagcagct 301 gcttccgcca gagtggaact tcctctggca gctgccacac acagcctgtt ctgttcctct 361 gaatctctgg gagctaggaa gtgggaccct tacccctttc accccacaac tccttcctgg 421 tcccctggcc cagcccctca tgactcctgt cagcccactc aattgtgact gtccctcctg 481 atgtattttt cttggcttaa agggtgtgtt aactcttttt acacttattt attagtatcc 541 tcatttctct ggaagccgca actggtgtca gcgc //