LOCUS BT007153 567 bp mRNA linear HUM 13-MAY-2003 DEFINITION Homo sapiens v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog mRNA, complete cds. ACCESSION BT007153 VERSION BT007153.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 567) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 567) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow Circle, Palo Alto, CA 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..567 /db_xref="H-InvDB:HIT000100073" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH00755X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..567 /codon_start=1 /product="v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog" /protein_id="AAP35817.1" /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQV VIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKR VKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLV REIRKHKEKMSKDGKKKKKKSKTKCVIM" BASE COUNT 208 a 80 c 134 g 145 t ORIGIN 1 atgactgaat ataaacttgt ggtagttgga gctggtggcg taggcaagag tgccttgacg 61 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 121 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 181 catgaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 241 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 301 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 361 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 421 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt 481 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag 541 tcaaagacaa agtgtgtaat tatgtag //