LOCUS BT006818 450 bp mRNA linear HUM 13-MAY-2003 DEFINITION Homo sapiens calmodulin 1 (phosphorylase kinase, delta) mRNA, complete cds. ACCESSION BT006818 VERSION BT006818.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 450) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 450) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow Circle, Palo Alto, CA 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..450 /db_xref="H-InvDB:HIT000099738" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH00517X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..450 /codon_start=1 /product="calmodulin 1 (phosphorylase kinase, delta)" /protein_id="AAP35464.1" /translation="MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNP TEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIS AAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK" BASE COUNT 163 a 76 c 112 g 99 t ORIGIN 1 atggctgatc agctgaccga agaacagatt gctgaattca aggaagcctt ctccctattt 61 gataaagatg gcgatggcac catcacaaca aaggaacttg gaactgtcat gaggtcactg 121 ggtcagaacc caacagaagc tgaattgcag gatatgatca atgaagtgga tgctgatggt 181 aatggcacca ttgacttccc cgaatttttg actatgatgg ctagaaaaat gaaagataca 241 gatagtgaag aagaaatccg tgaggcattc cgagtctttg acaaggatgg caatggttat 301 atcagtgcag cagaactacg tcacgtcatg acaaacttag gagaaaaact aacagatgaa 361 gaagtagatg aaatgatcag agaagcagat attgatggag acggacaagt caactatgaa 421 gaattcgtac agatgatgac tgcaaaatag //