LOCUS       BQ295201                 773 bp    mRNA    linear   EST 29-DEC-2010
DEFINITION  WHE2867_C11_E21ZS Wheat unstressed root tip cDNA library Triticum
            aestivum cDNA clone WHE2867_C11_E21, mRNA sequence.
ACCESSION   BQ295201
VERSION     BQ295201.1
DBLINK      BioSample: SAMN00170505
KEYWORDS    EST.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Anderson,O.D., Chao,S., Chin,A., Close,T.J., Crossman,C.,
            Gustafson,P., Lazo,G.R., Pham,J., Rausch,C.J., Ross,K., Wilson,C.
            and Woo,J.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes - Unstressed root tip cDNA library
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Email: oandersn@pw.usda.gov
            Sequences have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: SK primer.
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Triticum aestivum"
                     /mol_type="mRNA"
                     /cultivar="Chinese Spring"
                     /db_xref="taxon:4565"
                     /clone="WHE2867_C11_E21"
                     /tissue_type="Root tip at 1.0 to 1.5 mm stage"
                     /clone_lib="SAMN00170505 Wheat unstressed root tip cDNA
                     library"
                     /dev_stage="Four-day old seedling"
                     /lab_host="E. coli SOLR"
                     /note="Vector: Lambda Uni-ZAP XR, excised phagemid;
                     Site_1: EcoRI; Site_2: XhoI; Plants were grown under
                     hydroponic conditions for four days. Root tips were
                     excised and snap frozen (Ross and Gustafson) and total RNA
                     was prepared at University of Missouri, Columbia. Poly(A)
                     RNA was purified, a cDNA library was made, and the cDNA
                     clones were in vivo excised to give pBluescript
                     SK(-)phagemids in the TJ Close lab (Chin and Close) at the
                     University of California, Riverside. Plasmid DNA
                     preparations and DNA sequencing were performed in the OD
                     Anderson lab (all other authors)."
BASE COUNT          174 a          225 c          214 g          158 t
ORIGIN      
        1 tcggcacgag tccccgcagc caatcctccc caacccaagc tcgcagccat gcctcccaag
       61 ctcgacccgt cccagatcgt ggaggtgtat gtccgcgtca ccggcgggga ggtcggcgct
      121 gcgtcgtccc tggcccccaa gatcggtccg ctcggtctct ccccaaagaa gatcggagaa
      181 gacatcgcca aggagacggc caaggactgg aagggcctcc gcgtcaccgt caagctcacc
      241 gtccagaatc gccaggccaa ggtgtccgtc gtcccctccg ccgcagccct cgtcatcaag
      301 gcgctcaagg agcccgagag ggaccgcaag aaggtcaaga acatcaagca cagcggcaac
      361 atcagcctcg acgacgtcat cgagatcgcc aagatcatga aggtccgctc catggccaag
      421 gagatggccg gcaccgtcaa ggagatcctc ggcacctgcg tcagcgtcgg ctgcaccgtt
      481 gacggcaagg accccaagga tctgcagacg gagatcgacg acggcgaggt ggagatcccc
      541 gcttaagaag gcttggcttt gggcaggttg ttatgtgagg ctgctgaagt agtttctcct
      601 atctagatgc ttcctgatgc atttgagaga atggatgtga tatcttcact tgttctactc
      661 ttgctgaaga aganactttt gtcatgcttt actggttcca ttgtgagact attntttact
      721 ggtttaatga gtaccttgcg gaatcaatca atgttattat ggcagctaga ata
//