LOCUS BQ295201 773 bp mRNA linear EST 29-DEC-2010 DEFINITION WHE2867_C11_E21ZS Wheat unstressed root tip cDNA library Triticum aestivum cDNA clone WHE2867_C11_E21, mRNA sequence. ACCESSION BQ295201 VERSION BQ295201.1 DBLINK BioSample: SAMN00170505 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 773) AUTHORS Anderson,O.D., Chao,S., Chin,A., Close,T.J., Crossman,C., Gustafson,P., Lazo,G.R., Pham,J., Rausch,C.J., Ross,K., Wilson,C. and Woo,J. TITLE The structure and function of the expressed portion of the wheat genomes - Unstressed root tip cDNA library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequences have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: SK primer. FEATURES Location/Qualifiers source 1..773 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Chinese Spring" /db_xref="taxon:4565" /clone="WHE2867_C11_E21" /tissue_type="Root tip at 1.0 to 1.5 mm stage" /clone_lib="SAMN00170505 Wheat unstressed root tip cDNA library" /dev_stage="Four-day old seedling" /lab_host="E. coli SOLR" /note="Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-)phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 174 a 225 c 214 g 158 t ORIGIN 1 tcggcacgag tccccgcagc caatcctccc caacccaagc tcgcagccat gcctcccaag 61 ctcgacccgt cccagatcgt ggaggtgtat gtccgcgtca ccggcgggga ggtcggcgct 121 gcgtcgtccc tggcccccaa gatcggtccg ctcggtctct ccccaaagaa gatcggagaa 181 gacatcgcca aggagacggc caaggactgg aagggcctcc gcgtcaccgt caagctcacc 241 gtccagaatc gccaggccaa ggtgtccgtc gtcccctccg ccgcagccct cgtcatcaag 301 gcgctcaagg agcccgagag ggaccgcaag aaggtcaaga acatcaagca cagcggcaac 361 atcagcctcg acgacgtcat cgagatcgcc aagatcatga aggtccgctc catggccaag 421 gagatggccg gcaccgtcaa ggagatcctc ggcacctgcg tcagcgtcgg ctgcaccgtt 481 gacggcaagg accccaagga tctgcagacg gagatcgacg acggcgaggt ggagatcccc 541 gcttaagaag gcttggcttt gggcaggttg ttatgtgagg ctgctgaagt agtttctcct 601 atctagatgc ttcctgatgc atttgagaga atggatgtga tatcttcact tgttctactc 661 ttgctgaaga aganactttt gtcatgcttt actggttcca ttgtgagact attntttact 721 ggtttaatga gtaccttgcg gaatcaatca atgttattat ggcagctaga ata //