LOCUS BG088429 600 bp mRNA linear EST 12-MAY-2010 DEFINITION H3153A07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3153A07 5', mRNA sequence. ACCESSION BG088429 VERSION BG088429.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 600) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088429.1. Other_ESTs: H3153A07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3153 row: A column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..600 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3153A07-5" /db_xref="taxon:10090" /clone="H3153A07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 119 a 186 c 162 g 133 t ORIGIN 1 ctccgctcac actacgatgg catccgctcc ctggccttcc accacagcca gtcagcactg 61 cttactgcct ctgaggatgg cacactcaaa ctgtggaacc tgcagaaggc agttacggcc 121 aagaaaaatg ctgcactgta tgtggagccg gttcatgctt tccgagctca caggggcccg 181 gtgctggcag tcaccatggg cagcaacagc gagtactgtt acagtggtgg ggctgatgcc 241 aggatccaca gctggaagat tcctgacctc aacatggacc catatgatgg ctatgaccca 301 agtgtgctga gccatgtcct ggaaggccat ggggatgctg tgtggggtct ggccttcagt 361 cccacctccc agcgcctagc atcctgctct gccgatggca ccgtccgcat ctgggatccc 421 agcagcagtg gtccaagctg cctctgtacc ttccccatgg atggagaaca tggaattccc 481 acctcagtgg ccttcaccag cactgaacct gcccatgtcg tggcctcctt tcgttctggt 541 gataccgttc tttatgacct ggaagctggc agcgcccttc tcacattgga gtctcgaggg //