LOCUS BG088015 693 bp mRNA linear EST 12-MAY-2010
DEFINITION H3147F01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3147F01 5', mRNA sequence.
ACCESSION BG088015
VERSION BG088015.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 693)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG088015.1.
Other_ESTs: H3147F01-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3147 row: F column: 01
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..693
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3147F01-5"
/db_xref="taxon:10090"
/clone="H3147F01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 221 a 135 c 164 g 173 t
ORIGIN
1 ttaaacagag aaagcatgat gaagtccgga tggtcgattt ctattctcca tggtgtcatc
61 cctgtcaggt cttgatgcca gaatggaaac gcatggcccg gacgttaact ggactgatca
121 atgtaggcag tgtggactgc caacagtatc attctttttg tactcaagaa aatgttcaaa
181 gataccccga gataagattt tatccccaaa agtcaagtaa agcctatcag tatcatagtt
241 acaatggctg gaatagggat gcgtattccc tgagaagctg gggtcttgga tttttgcccc
301 aagcatccat agatttaaca cctcagactt tcaatgaaaa agttctacaa gggaaaactc
361 actgggtggt tgatttctat gctccttggt gtggaccttg ccagaatttt gctcctgagt
421 ttgaactctt ggctaggatg atcaaaggaa aagtgagagc cgggaaagtg gactgtcagg
481 cttaccccca gacgtgccag aaagccggca tcaaagccta ccccagtgtc aaactgtacc
541 agtacgagag agcaaagaaa agtatttggg aagagcaaat aaattccaga gatgcaaaaa
601 ctattgctgc cttaatttat ggaaaattgg aaacgctcca aagccaagta aaaagaaata
661 aggatgagct ttgatggtgt tgaagaaaaa gca
//