LOCUS BG086559 661 bp mRNA linear EST 12-MAY-2010 DEFINITION H3127G05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3127G05 5', mRNA sequence. ACCESSION BG086559 VERSION BG086559.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 661) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086559.1. Other_ESTs: H3127G05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3127 row: G column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..661 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3127G05-5" /db_xref="taxon:10090" /clone="H3127G05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 196 a 151 c 135 g 179 t ORIGIN 1 ctgagtggtt tggattttac agaagtggcc aagctaagga aaccattccc ctccaggaga 61 gcactctata cacagaggac cgcctggggc taaagaaaat ggacaaagca ggaaagctag 121 tgtttctggc taaggaaggg gaccatcttc aaatatctaa agaatggttt actgcccaca 181 tcataccttt tcttaagtga agccctggca ctttatagca gagttcatga aaccacagct 241 cttccaagcc atgtacatag ttcatgctca gccctgaact ctaatctagc ctgcaaccag 301 cccttctctc ctcttatcat ctaacatacc ctacttggaa agatctaaga tctcaatctt 361 atcctttgcc gcctgctatc accatatggt gttggattca agtttaatta gctaataacc 421 atggagattg ttttacaact ttgtgatatg gtcaagctcc cgtttaagaa atgggagtct 481 gttgttgcag atcagtaatt gtgcctaaaa gagacagaag tgaaaaagag acaagctctg 541 agggcaaaac ccatacaaat ttggagcaaa taaaagccca ggtctgagat gttcacacaa 601 gcaatttctc tgcactgctg tgtgggccgt gcatgatgtt tccccatcat ttcttaagac 661 t //