LOCUS       BG085363                 465 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3112D08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3112D08 5', mRNA sequence.
ACCESSION   BG085363
VERSION     BG085363.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12567927.
            Other_ESTs: H3112D08-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3112  row: D  column: 08
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3112D08-5"
                     /db_xref="taxon:10090"
                     /clone="H3112D08"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          138 a          123 c          108 g           96 t
ORIGIN      
        1 tttagaaagc aaaacttacc atgctctgag taatctgccg aaagcccgag ctgccttaac
       61 ctctgctcga accacagcaa atgccatcta ctgcccccct aaattgcagg ccaccctgga
      121 catgcagtca ggcattattc atgcagcaga ggagaaggac tggaaaactg catactcata
      181 cttctatgag gcattcgaag gctacgactc cattgatagc cccaaggcca tcacatctct
      241 gaagtacatg ttgctgtgca aaatcatgct caacacccca gaagatgtcc aggctttggt
      301 gagcggaaag cttgcacttc ggtatgcagg gaggcagaca gaagcattga aatgtgtggc
      361 tcaagctagc aagaacagat cactggcaga ttttgaaaag gccctgacag actacagggc
      421 agagctccgg gatgacccaa tcatcagcac acatttggcc aagct
//