LOCUS       BG085178                 605 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3109E02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3109E02 5', mRNA sequence.
VERSION     BG085178.1
DBLINK      BioSample: SAMN00163148
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3109E02-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            This clone set has been freely distributed to the community. Please
            visit for details.
            Plate: H3109  row: E  column: 02
            Seq primer: -21M13 Reverse
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Mus musculus"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          170 a          150 c          140 g          144 t
        1 tgattgcatg tctgtgattc taacacccag gaggaggatt gctgaaaggt tgggaccagc
       61 ctaagctcta gagtaagagc tgcctcaaac acatgcaaaa aaaaaaagca ctgactgccc
      121 ttccagagga cccaggtttg agtcccagca tcccatagca gctcacaact gcctgtaact
      181 tcagttccag gatatctcct tttgatctct gaggaccacg tgcattcatg gcacacagac
      241 ggacatgcag gcaaaataat ggcacatata aaaaagtcaa tctaaacaac aacaaaacaa
      301 aatacacaga gttgtactgg ctggttttac gtatcaactt gacacaagct ggagtcatca
      361 gagaggaaga agtctcagtt gaggaaatgc ctccttgaga tccagctgta gggcactttc
      421 tcaactagtt atcattgggg taggtcccag cccactgtgg gaggtgccat ccctgggctg
      481 gtatcttggg ctctataaga aagcaggctg agcaagccag taagcaacac ccttctgtgg
      541 cctctgcatc agcttctgcc tccaggttcc tgcagngttt gacttcctgt cctgatttct
      601 cagtg