LOCUS BG085119 576 bp mRNA linear EST 12-MAY-2010 DEFINITION H3108H01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3108H01 5', mRNA sequence. ACCESSION BG085119 VERSION BG085119.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 576) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085119.1. Other_ESTs: H3108H01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3108 row: H column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..576 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3108H01-5" /db_xref="taxon:10090" /clone="H3108H01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 172 a 105 c 104 g 195 t ORIGIN 1 agacatcact tttcttgaat agcaaagctg ggagagtgac cagctcactg tatgaacacg 61 gacattgctg actggtaatg aagtgccctc atgtgttact ctcctggctc tttcatccct 121 tgctctagaa agcccctgta atttaattaa cagactgcct gtaggtatag tgcaattatg 181 aatgctctga tcgttgtaca tacatctctc ttgatattgc aacatccata ctggctttgt 241 aatcattaat tttttggcag attgaatgtg ctgtattgat atgtatctat gtaattgtat 301 gtcttatagc taattcacat tttgaataat gttattttat ttactttttt aagagaggag 361 aatgtaaatt tgtcagttta tttctgacta gggatatttt tttccattta gaaaagaaga 421 aaaaaaaaaa aacccttact attgtacaga gcggtactag catcgtgctg tctaaaatca 481 tttgcacatt cctgagtaga ggtgtgctga cctaagaccc aaaggtaatt tcatagcaaa 541 cacacaccat ccgtcagagc ttttatacaa acacgg //