LOCUS BG085088 649 bp mRNA linear EST 12-MAY-2010 DEFINITION H3108E03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3108E03 5', mRNA sequence. ACCESSION BG085088 VERSION BG085088.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 649) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085088.1. Other_ESTs: H3108E03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3108 row: E column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..649 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3108E03-5" /db_xref="taxon:10090" /clone="H3108E03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 177 a 152 c 158 g 162 t ORIGIN 1 cctctagcca aagaccagag gcagcaggat tctctcctga ctgatgcttc tggaaaacca 61 aggacttgct cccttcttcc ccaggagctg ccccgtgttt agaagcggca gcagcagcag 121 caacaaccat agcggcggtg gcagcggcgg gggatgagtg acagagagcg tcctatgcct 181 tggagactgt catggcataa gctgtgctag cacctcctca ggaaatgaga ttgattttta 241 caacagccaa taacgttgca ctttattaat gcctgtgtgt aaatacgaat agctatgttt 301 tatatatatc tatatatcta tatgtctata tctctctata tatagccata ctctgcaagg 361 agacaaagaa aatgatcaaa tgtgttcccc ggggaattag tttttattgg agagctctag 421 aatggagcag aagggactcg ggatagcgtt agcacttgac aatcagtcac acaagcaacg 481 atcccctgac agcagggttg ggggcacaat tgtatgagaa ggatccatgc cttgcagcct 541 gctttggcca caaaacactt tgttttgcaa taatgaccct ctacagtagg gtgctttatg 601 gaccagggag ctgagctcca gtccagcact gagtcccagg atctcccat //