LOCUS BG085011 569 bp mRNA linear EST 12-MAY-2010 DEFINITION H3107F04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3107F04 5', mRNA sequence. ACCESSION BG085011 VERSION BG085011.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 569) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085011.1. Other_ESTs: H3107F04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3107 row: F column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..569 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3107F04-5" /db_xref="taxon:10090" /clone="H3107F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 133 a 161 c 154 g 121 t ORIGIN 1 ccggcgggaa gggacctagg gcttgctccg cttcgcctct gctggaagga aggactttat 61 cacaaagcag gcgcctaggg ctaaggaaag ccagggtgtc ttgcttcttt gagccggaca 121 gcagggagga ggccccttct tctgcatatt tgcttcctgg gacgcggctc cagagaggac 181 ccttcaaaca cgttgggttg gttcaaaaag aattggcacc ccagagacct ctgccaactg 241 ccagactccg agagagtgaa ccacctgcct ttcaaccctc ccgccacgtg tgtctgagga 301 gactcgggtt tggttgcttt ggataccaag aacacgagct tgtcggatat gaagacagaa 361 gaaatggtga cccaccccat ggaggtacac agccagcgat cacttcttat agattacaga 421 cgaagcctga gaacacacct gctgtctttg gggacatggg cttcctgcgg tgctccagtt 481 ggaggaaata cagtccacga ccccaggctg cctggcaggc tctctgaact gccccaatat 541 cctctgacct agtcaatcca ttccagcat //