LOCUS BG084487 606 bp mRNA linear EST 12-MAY-2010 DEFINITION H3101E03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3101E03 5', mRNA sequence. ACCESSION BG084487 VERSION BG084487.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 606) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG084487.1. Other_ESTs: H3101E03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3101 row: E column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..606 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3101E03-5" /db_xref="taxon:10090" /clone="H3101E03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 133 a 195 c 152 g 126 t ORIGIN 1 catgctccca cgtgtccgtg ggccgacatc tacctgtgtc taggctctgc caccttcagg 61 attcacaact gtgtcttctc tcaggacctg agaacccacg tccccatcac agatgtcaca 121 tgtgtgctag tccagaaata gaagaacatt tgtcagctcc gacacaggcc tctcagccac 181 cttcctgaac aagttgagca tggccagcct gtggcaatgc cctcaggtgt ggatgcgggc 241 tcctgaggtg ttggggtgac tgacgactga ggtcacttca cacatggcca gcgtgagccg 301 acaccaagcc ttgcctcagc ctccacctgt ggggctccca ctaatttcct cctggatgga 361 acgactcggg cactgttgtc atcagagtga cagccctgga gccttgccag tgacttcctc 421 gcctcacatg gacccccagg ttcactgcac acgctgagaa atagaattga gaagtaaagg 481 aaatggcctt caaaaggcca cacctgagac ccgcccctgc tttatgacac ccttccctgg 541 gcctcatgct gtaccacagg aggcagagtc agcacagccg tggccagggc acccccaggt 601 ccctga //