LOCUS BG082840 588 bp mRNA linear EST 12-MAY-2010 DEFINITION H3081C12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3081C12 5', mRNA sequence. ACCESSION BG082840 VERSION BG082840.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 588) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082840.1. Other_ESTs: H3081C12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3081 row: C column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..588 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3081C12-5" /db_xref="taxon:10090" /clone="H3081C12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 141 a 163 c 168 g 116 t ORIGIN 1 gtgcactcct ggcagctgtt gggactttgt ggagaccaag agctgcacgc ttgaaactgc 61 agtttcaaca gaagaaccct gtctcgctga agcagagggt agaaagagcg actggtagac 121 ctgagagaac actacctctc cggtaaaggg ctccagacaa ggattctaaa ttcagcttcc 181 cagaggtgac aatcgatcgc tgggagcaaa gtttggattc aagcgctctg gaagatactt 241 aagtacccaa atattcctag tccagtcttc gctctgggag aagggcattc aagacgccct 301 ctcaaagagg cttcacctcc aacccatggc gctgaccagc tcctcacaag cagagacttg 361 gagcctgcat cctcgggctt ccacggcctc tttgccttta ggaccccagg agcaggaggc 421 cggcgggagc cctggagcgt ctgggggact tccgctggag aaggtgaagc ggcccatgaa 481 cgccttcatg gtgtggagct ctgttcagcg ccgccagatg gcgcagcaga accccaagat 541 gcacaactct gagatctcca agcgcttggg cgctcagtgg aagctgct //