LOCUS       BG077998                 582 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3022D12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3022D12 5', mRNA sequence.
ACCESSION   BG077998
VERSION     BG077998.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3022D12-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3022  row: D  column: 12
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3022D12-5"
                     /db_xref="taxon:10090"
                     /clone="H3022D12"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          141 a          154 c          157 g          130 t
ORIGIN      
        1 cgggagttct gtccgccgct gccggtgccg cggtgtcgcc gggggccacg gtgttgccgg
       61 gcaggatgag tgtgatagac cacgtgcggg acatggcggc tgcgggactg cactccaacg
      121 tgcggctcct ctcagtctgc tgctcaccat gagtaacaac aaccctgagc tgttttcccc
      181 gtcgcagaag taccagctct tggtgtatca tgcagactct ctgtttcacg ataaggagta
      241 ccgcaatgct gtgagtaagt acgccatggc tttacagcag aagaaagcct taagtaagac
      301 ttccaaagtg cggccttcca ctgggaattc tgcatctact ccacagagtc agtgtcttcc
      361 atctgaaatt gaagtgaaat acaaaatggc tgagtgttac acaatgctga agctagacaa
      421 ggatgccatt gctgtactcg atgggatccc ttcaagacaa agaactccca aaataaacat
      481 gatgctggca aacctgtgca agaaggctgg tcaggagcgc ccatcagtca caagctataa
      541 ggaggtcctg cggcagtgcc ctttggccct tgacgccatt ct
//