LOCUS BG076875 623 bp mRNA linear EST 12-MAY-2010 DEFINITION H3007E08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3007E08 5', mRNA sequence. ACCESSION BG076875 VERSION BG076875.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 623) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076875.1. Other_ESTs: H3007E08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3007 row: E column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..623 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3007E08-5" /db_xref="taxon:10090" /clone="H3007E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 169 a 196 c 135 g 123 t ORIGIN 1 aacagcccgg gaccatgctg tcgctccgct ccttgcttcc acacctggga ctgttcctgt 61 gcctggctct gcacttatcc ccctccctct ctgccagtga taatgggtcc tgcgtggtcc 121 ttgataacat ctacacctcc gacatcttgg aaatcagcac tatggctaac gtctctggtg 181 gggatgtaac ctatacagtg acggtccccg tgaacgattc agtcagtgcc gtgatcctga 241 aagcagtgaa ggaggacgac agcccagtgg gcacctggag tggaacatat gagaagtgca 301 acgacagcag tgtctactat aacttgacat cccaaagcca gtcggtcttc cagacaaact 361 ggacagttcc tacttccgag gatgtgacta aagtcaacct gcaggtcctc atcgtcgtca 421 atcgcacagc ctcaaagtca tccgtgaaaa tggaacaagt acaaccctca gcctcaaccc 481 ctattcctga gagttctgag accagccaga ccataaacac gactccaact gtgaacacag 541 ccaagactac agccaaggac acagccaaca ccacagccgt gaccacagcc aataccacag 601 ccaataccac agccgtgacc aca //