LOCUS BG075201 655 bp mRNA linear EST 12-MAY-2010 DEFINITION H3144E03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3144E03 3', mRNA sequence. ACCESSION BG075201 VERSION BG075201.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 655) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075201.1. Other_ESTs: H3144E03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3144 row: E column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..655 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3144E03-3" /db_xref="taxon:10090" /clone="H3144E03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 150 a 165 c 210 g 130 t ORIGIN 1 ctggaaaatg aggttttata atcttagctg tggccacaac ctagcagcac agagcaaggt 61 gggtgtccag gctgctgaag ccacgctcct ggagggttag ggcttctacc ctcagcaagc 121 acattgcata ggctgctcag gccagccaag ggcattggtg cagcagtgaa agcagcagca 181 ctatatgtga cccttgggtt ggggacctca gaatgatggg tgatccctgc agaagaggca 241 agggttggag cccagggctg ggcctcagca ccaggaggga gcctcaatac ttgtaggcca 301 tgctgaggcc ctgggtcagg gcacttgaga ctcgggaggg gattggaatg cagggagggt 361 agaggcaagg actatcctca gtgtgggtgg tccacggtgg gggacggacc tgtggtctgg 421 aaggaggtcc atcccaactc aggtctgggc tctagcggag tggcacttcc gacaaaggac 481 gtggccatcc agagggaaac agccgttgtc atctgcctca atggacagag gtttcccaca 541 gtcctcacac ttgtaacact tcatatgaaa gttcttatcc agcgccacta ctcgcacagt 601 ctcgtctcgg ccaggctcag gcatgattgg ctccgagcag acggagcacc ttgga //