LOCUS       BG074466                 540 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3135E03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3135E03 3', mRNA sequence.
ACCESSION   BG074466
VERSION     BG074466.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12557035.
            Other_ESTs: H3135E03-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3135  row: E  column: 03
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3135E03-3"
                     /db_xref="taxon:10090"
                     /clone="H3135E03"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          198 a           93 c           77 g          172 t
ORIGIN      
        1 ataatcaagg taaaattttt aataattttt taaaaaataa ctattctatg tattaaatac
       61 tctttactaa ttatacataa gatacacctt tgggcttaaa atcttatgag gaaaaacaca
      121 caattcaaaa gcattatcaa ataacacata tttaaagctt ttaatttttt tttaagggga
      181 aaagcaagag ataaaacaat taaaaagatg agatggtcag gtcatcttag ttcttgtctt
      241 ccattttact aactgtaata atttcttctc tttggattgt tcttttaaaa atgtattgct
      301 ttgcaaataa aattacaaca acaaaaacac ccaacttttt gagcaaacaa aacagaaaac
      361 atcccatgtt cagggacttc ttaggagctc tgaagagtag acatggtttt tttcaacgac
      421 gatgcaaaca tgtcaacccg gtgaaatcag cactgtgcct ggttcccaca tgttcacaca
      481 cagagtgaca cacacagagt ttatgctgcg gactcaagag aatatttcca gttttgacct
//