LOCUS BG074384 606 bp mRNA linear EST 12-MAY-2010 DEFINITION H3134D12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3134D12 3', mRNA sequence. ACCESSION BG074384 VERSION BG074384.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 606) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074384.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3134 row: D column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..606 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3134D12-3" /db_xref="taxon:10090" /clone="H3134D12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 190 a 124 c 112 g 180 t ORIGIN 1 gagagagaaa taaatgttta ttggcatatc attttgattt tggaagtttg tctggagtga 61 cttctaatat aaatgattca atttttatca ttttgagttg tatacttcaa gacaagtctc 121 ttgctttatc aaatgtgaaa acatgcccca tcctgtgcca agaaatatgc tgcatcccaa 181 gtttctttaa acatccacag agacggtggc taatgtccat tctgtcctca caatatcccc 241 gtattgtctt cagtttctag gggaggaaac agagacacag aaaagttaag aaatttgcct 301 ggagtcgcac agaaaggacc aagcaacgtt accaggttta gcagactaca gcaaacagga 361 catatttaaa gcaaagaaat tatacattgt tcctctaagt tctaatttaa ctgggtttcc 421 tatgtcttat ctgccaaatc tgttaggagg gtaccccaga atttgaaacc caacaatctg 481 accacaagct tcttgactca cttcattttt ctttttcatt atacatgaaa gcagactcat 541 gcaaacagag aaatcacagt gacatctggc cagtgccctg gagccacagc ttcaggcatg 601 taatgg //