LOCUS BG073439 618 bp mRNA linear EST 12-MAY-2010 DEFINITION H3122F03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3122F03 3', mRNA sequence. ACCESSION BG073439 VERSION BG073439.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 618) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073439.1. Other_ESTs: H3122F03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3122 row: F column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..618 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3122F03-3" /db_xref="taxon:10090" /clone="H3122F03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 172 a 155 c 166 g 125 t ORIGIN 1 gtttgcttta aggatcttta tttaagtgcc tacggtgttg aagttccatc aaggatttgg 61 aaggtcactg tcccaccact cctgccagtc agtgatgagt ggctggcagc cggatccgtg 121 gtgttcatac ataaggcagg agaatagaga atccagcttt tccagatggt tcagctgcag 181 ttctgggacc ctcgagtccc ggctccaagg ctgagaggat gtacagcttc gaggtggcca 241 gcttcctaga gtcaccactg tgcttcagtc catggtgaga ggacagtcac cggctccagt 301 tcacaacccg cccctctggc taatggcaga aggatgagag aagccagccg aaaaggtgaa 361 attaatggat cccaggagca cagcacacct cagatgctgg cttagggctg aaagaaccag 421 tgtcaagtca ggctcctgag ccagggtgga tcttcccacc taccgccatc accacaaccc 481 cagtcagaac tggagaacct gacagtggtg gagctggcca aggtacgagt tttcatccaa 541 agcaatggag cagggcatta gacaggcaaa tacacaaaca aaccctagga caaagatgaa 601 aaggaactga gataacaa //