LOCUS BG072915 551 bp mRNA linear EST 12-MAY-2010 DEFINITION H3116E02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3116E02 3', mRNA sequence. ACCESSION BG072915 VERSION BG072915.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 551) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG072915.1. Other_ESTs: H3116E02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3116 row: E column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..551 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3116E02-3" /db_xref="taxon:10090" /clone="H3116E02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 133 a 155 c 152 g 111 t ORIGIN 1 ccaaatttga ggtgtattta catatcaaat tgggctcagt ggaccacagt agatgggacc 61 caaacaggag gggcaatatc agcctatacc ctatctctct cttccccttt gaggaggagc 121 ccagcttcct acccagggcc tgcccaagga gaggggagag gcacaaacat gcgcaggcac 181 tggcccgcag ccccagcctg tgcagaagaa gaagggagac agggctcagg tggccaagtt 241 gtcggccggg aggctggaca tgcggagctg ggagctgctc ttgctcagga tggacagctg 301 ggtgcccccg ttcactccac tgctggccag ggatggatgc cggtgcttga agtccatagt 361 gaagtaacgg ttcaccttcc agctcctcca tttcctctta atctctgcct gtacctcccc 421 attcaggaag cagtagagta cagccaccac aaagccctgg aaggagccca gcccaagctc 481 aaacacaagt ctttccctct tgctgacgtt ctctggagag aaggcaaata ctgtgtagtg 541 gattccaaag a //