LOCUS BG071642 627 bp mRNA linear EST 26-JAN-2001 DEFINITION H3101E03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3101E03 3', mRNA sequence. ACCESSION BG071642 VERSION BG071642.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 627) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3101E03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3101 row: E column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..627 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3101E03-3" /db_xref="taxon:10090" /clone="H3101E03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 132 a 162 c 191 g 142 t ORIGIN 1 cttgcaaaag tattttatta atacgcttgc gatgtacaaa tggaaagtaa atatgtgatt 61 ctacctggtc agctgactgg gccacggggc cctgtctgtg ctcagagtct accttggtgc 121 ttgggacgga gacctggcag tagctgagtg gcgggctgct cagtgcacag cactgggggg 181 aggggtgcac tgctagagta ccatgagtca aggacctggc gggtgccctg gccacggctg 241 tactgactct ccctcctgtg gtacagcatg aggcccaggg aagggtgtca taaagcacgg 301 gcgggtctca ggtctggcct tttgaaggcc atttccttta cttcgcaatt ctatggctca 361 gcgtgtgcag tgaacctggg ggtccatgtg agcgcgatga agtcactggc aaggctccag 421 ggctgtcacg ctgatgacaa cagtgcccaa gtcgttccat ccaggaggaa attagtgaga 481 tccccacagg tgcatgctga ggcaaggctt ggtgtcggct cacgctggcc atgtatgaag 541 tgacctcagt cgtcagtcac cccaacacct caggagcccg catccacacc tgagggcatt 601 gcacaggctg gccatgctca acttgtt //