LOCUS BG068801 759 bp mRNA linear EST 12-MAY-2010 DEFINITION H3069D12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3069D12 3', mRNA sequence. ACCESSION BG068801 VERSION BG068801.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 759) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068801.1. Other_ESTs: H3069D12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3069 row: D column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..759 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3069D12-3" /db_xref="taxon:10090" /clone="H3069D12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 194 a 187 c 194 g 184 t ORIGIN 1 ctgaaaatat cttgcctttt ttattaatca tgtcatctaa caccaattaa aaaaacaagt 61 atgtgaatat gatataaaaa tacgatccca agattaacac ttgtcgcagg cacaagttat 121 cgcacagaat acatgtttgg agggccggga aaggagtctg gttctgtttc tctttaaaaa 181 acccacagca gtgcaagtag ggtgagggag gggagattgt ttagcagcaa aatgtccgag 241 ttctctattc acatcgaaaa aacaaaacaa aacaaaacgt aagctcaggg gaagctgccc 301 agacccactt gtcactaaac acttccgctg ccacagcccc aggtagcgac aggcgaaggg 361 tgcgctttac tacacaagcc atcgtaaact cgtccctcaa gctttgactg ttgtcactta 421 aatatatgta caggaatcct cttctcgtca tcccctcaga tctccactgt ttggtcacac 481 gggtgggctc tcaggatctg gccacatctg gttacaggct ggagctgcag cctcgaggca 541 gatgctgctg cggcctggct ggctggcgtg gcggcttccg gcggaggagc gatggggcct 601 cagtggtcac tgctctgttt ctctctcatc caggcctgaa tctgctcctt tagctctggc 661 actggctcca gcatgctctc agtcagcatc tggcggttga aagggtcggt gggggagttg 721 agcagatgcc gcaggatgat agaacggtcc atgacggtg //